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Polymorphism of the alleles of the merozoite surface antigens MSA1 and MSA2 in Plasmodium falciparum wild isolates from Colombia
- Source :
- Molecular and Biochemical Parasitology. 49:265-275
- Publication Year :
- 1991
- Publisher :
- Elsevier BV, 1991.
-
Abstract
- The degree of polymorphism and the allelic distribution of 2 major Plasmodium falciparum merozoite surface antigens (MSA1 and MSA2) have been analysed in clinical isolates from Colombia. DNA was prepared directly from patients' blood and used in PCR reactions to amplify block 2 of MSA1 and the central region from MSA2. Thirty one samples were analysed and a marked degree of length polymorphism was detected, especially for MSA2. A high proportion of multiple bands was also observed, most probably resulting from mixed infections. Allele-specific oligonucleotides were used to type both alleles. For MSA1, 26 out of 31 clinical isolates were of the RO33 type, 15 were MAD20 and three were typed as KI. When the MSA2 allele was analysed, 7 isolates hybridised with a CAMP specific probe and 6 hybridised strongly with an FC27-derived oligonucleotide. Two samples, which showed multiple bands, hybridised with both probes. Interestingly, in 14 out of 27 isolates the MSA2 allele remained unassigned by the specific probes. Five of these were cloned and their DNA sequenced; these sequences are discussed.
- Subjects :
- Adult
Male
Adolescent
Sequence analysis
Molecular Sequence Data
Plasmodium falciparum
Protozoan Proteins
Antigens, Protozoan
Biology
Molecular cloning
Polymerase Chain Reaction
law.invention
law
Animals
Humans
Cloning, Molecular
Malaria, Falciparum
Protein Precursors
Allele
Child
Molecular Biology
Alleles
Merozoite Surface Protein 1
Polymerase chain reaction
Genetics
Cloning
Polymorphism, Genetic
Base Sequence
Oligonucleotide
Nucleic acid sequence
DNA, Protozoan
Middle Aged
South America
biology.organism_classification
Molecular biology
Antigens, Surface
Female
Parasitology
Sequence Alignment
Subjects
Details
- ISSN :
- 01666851
- Volume :
- 49
- Database :
- OpenAIRE
- Journal :
- Molecular and Biochemical Parasitology
- Accession number :
- edsair.doi.dedup.....ffa0a0c1c5fc964e0505282f071042bf