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Coupled sterol synthesis and transport machineries at ER–endocytic contact sites
- Source :
- Digital.CSIC. Repositorio Institucional del CSIC, instname
- Publication Year :
- 2021
- Publisher :
- Rockefeller University Press, 2021.
-
Abstract
- Sterols are unevenly distributed within cellular membranes. How their biosynthetic and transport machineries are organized to generate heterogeneity is largely unknown. We previously showed that the yeast sterol transporter Osh2 is recruited to endoplasmic reticulum (ER)–endocytic contacts to facilitate actin polymerization. We now find that a subset of sterol biosynthetic enzymes also localizes at these contacts and interacts with Osh2 and the endocytic machinery. Following the sterol dynamics, we show that Osh2 extracts sterols from these subdomains, which we name ERSESs (ER sterol exit sites). Further, we demonstrate that coupling of the sterol synthesis and transport machineries is required for endocytosis in mother cells, but not in daughters, where plasma membrane loading with accessible sterols and endocytosis are linked to secretion.
- Subjects :
- Saccharomyces cerevisiae Proteins
Endocytic cycle
Saccharomyces cerevisiae
Biology
Endoplasmic Reticulum
Endocytosis
03 medical and health sciences
0302 clinical medicine
polycyclic compounds
Secretion
Actin
030304 developmental biology
0303 health sciences
Trafficking
Endoplasmic reticulum
Cell Membrane
Biological Transport
Transporter
Cell Biology
Sterol
Yeast
Cell biology
Sterols
lipids (amino acids, peptides, and proteins)
Carrier Proteins
Membrane and lipid biology
030217 neurology & neurosurgery
Subjects
Details
- ISSN :
- 15408140 and 00219525
- Volume :
- 220
- Database :
- OpenAIRE
- Journal :
- Journal of Cell Biology
- Accession number :
- edsair.doi.dedup.....ff9d96cc718ecf565e3529fe09f799a0