Back to Search
Start Over
Fabrication of graphene oxide and sliver nanoparticle hybrids for fluorescence quenching of DNA labeled by methylene blue
- Source :
- Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy. 243
- Publication Year :
- 2020
-
Abstract
- Since graphene oxide‑silver nanoparticles (GO-AgNPs) have special affinities to DNA, it become increasingly important in fields of biological analysis in which GO-AgNPs nanocomposites universally functioned as a quencher. In this paper, GO-AgNPs nanocomposites with different GO to AgNPs ratios were synthesized as a fluorescence quencher to interact with DNA labeled by methylene blue (MB). The results showed that the fluorescence intensity of DNA-MB system decreased with the increasing of GO-AgNPs nanocomposites concentration. The quenching phenomenon of DNA-MB by AgNPs and GO was not a simple additive effect but a synergistic effect. The quenching efficiency of synthesized GO-AgNPs nanocomposites with different ratios (1:1, 1:3, 1:5, 1:10) increased with the decrease of GO/Ag ratio. Thermodynamic analysis was employed to investigate the interaction of GO-AgNPs and DNA-MB, it can be concluded that the intermolecular force between GO-AgNPs and DNA-MB was hydrogen bonding. Our works will provide important theoretical and experimental bases for fluorescence sensing of DNA.
- Subjects :
- Silver
Oxide
Nanoparticle
Metal Nanoparticles
02 engineering and technology
010402 general chemistry
Photochemistry
01 natural sciences
Fluorescence
Analytical Chemistry
law.invention
chemistry.chemical_compound
law
Instrumentation
Spectroscopy
Quenching (fluorescence)
Nanocomposite
Graphene
Chemistry
Hydrogen bond
Oxides
DNA
021001 nanoscience & nanotechnology
Atomic and Molecular Physics, and Optics
0104 chemical sciences
Methylene Blue
Graphite
0210 nano-technology
Methylene blue
Subjects
Details
- ISSN :
- 18733557
- Volume :
- 243
- Database :
- OpenAIRE
- Journal :
- Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy
- Accession number :
- edsair.doi.dedup.....ff91adfd880a83a04239140c1e171382