Sequence analyses and chromosomal distribution of the Tc1/Mariner element in Parodontidae fish (Teleostei: Characiformes)

Made available in DSpace on 2018-11-27T21:47:44Z (GMT). No. of bitstreams: 0 Previous issue date: 2016-11-30 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Secretaria de Ciencia e Tecnologia do Estado do Parana (SETI) Fundacao Araucaria de Apoio ao Desenvolvimento Cientifico do Estado do Parana (Fundacao Araucaria) Transposable elements are able to move along eukaryotic genomes. They are divided into two classes according to their transposition intermediate: RNA (class I or retrotransposons) or DNA (class II or DNA transposons). Most of these sequences are inactive or non-autonomous in eukaryotic genomes. Inactivate transposons can accumulate mutations at neutral rates until losing their molecular identity. They may either be eliminated from the genome or take on different molecular functions. Transposable elements may also participate in the differentiation of sex chromosomes. Therefore, the structural variations and nucleotide similarity of Tc1/Mariner sequences were analyzed along with their potential participation in the differentiation processes of sex chromosomes in the genomes of Parodontidae fish. All Parodontidae species presented non-autonomous copies of Tc1/Mariner with structural variation, different levels of deterioration (genetic distance), and variations in insertion and deletion patterns. The physical mapping of Tc1/Mariner on chromosomes revealed dispersed signals in euchromatins, with small accumulations in terminal regions and in the sex chromosomes. The gene dosage ratios indicated copy number variations of Tc1/Mariner among the genomes and high transposase open reading frame deterioration in Parodon hilarii and Parodon pongoensis genomes. This transposon presented transcriptional activity in gonads, but there was no significant difference between sexes. This may indicate non-functional protein expression or may correspond to DNA binding proteins derived from Tc1/Mariner. Thus, our results show Tc1/Mariner inactivation along with a diversity in Parodontidae genomes and its participation in the differentiation of the W sex chromosome. (C) 2016 Elsevier B.V. All rights reserved. Univ Fed Parana, Dept Genet, Curitiba, Parana, Brazil Univ Estadual Ponta Grossa, Dept Struct & Mol Biol & Genet, Ponta Grossa, Parana, Brazil Sao Paulo State Univ, Dept Bioproc & Biotechnol, Botucatu, SP, Brazil Sao Paulo State Univ, Inst Biosci, Dept Morphol, Sao Paulo, Brazil Univ Fed Sao Carlos, Dept Genet & Evolut, Sao Carlos, SP, Brazil Sao Paulo State Univ, Dept Bioproc & Biotechnol, Botucatu, SP, Brazil Sao Paulo State Univ, Inst Biosci, Dept Morphol, Sao Paulo, Brazil CNPq: 303696/2013-3 CNPq: 473212/2013-7 CAPES: 40001016006 FAPESP: 2012/15258-0 FAPESP: 2013/04533-3 Secretaria de Ciencia e Tecnologia do Estado do Parana (SETI): 378/2012 Fundacao Araucaria de Apoio ao Desenvolvimento Cientifico do Estado do Parana (Fundacao Araucaria): 22.843