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Identification of circular RNAs in the ovarian follicles of Meishan and Duroc sows during the follicular phase
- Source :
- Journal of Ovarian Research, Journal of Ovarian Research, Vol 13, Iss 1, Pp 1-14 (2020)
- Publication Year :
- 2020
-
Abstract
- Circular RNAs (circRNAs) are a newly discovered class of endogenous non-coding RNAs that play an important role in growth and development by regulating gene expression and participating in a variety of biological processes. However, the role of circRNAs in porcine follicles remains unclear. Therefore, this study examined middle-sized ovarian follicles obtained from Meishan and Duroc sows at day 4 of the follicular phase. High-throughput RNA sequencing (RNA-seq) was utilized to construct circRNAs, and differential expression was identified. The findings were validated using reverse transcription PCR (RT-PCR) and DNA sequencing, GO and KEGG analyses were performed, and potential miRNA targets were identified. The RNA-seq identified a total of 15,866 circRNAs, with 244 differentially expressed in the Meishan relative to the Duroc (111 up-regulated and 133 down-regulated). The RT-PCR finding confirmed the RNA-seq results, and quantitative real-time PCR (qPCR) analysis examining a subset of the circRNAs showed that they are resistant to RNase R digestion. Bioinformatics analysis (GO and KEGG) showed that the host genes associated with the differentially expressed circRNAs are involved in reproduction and follicular development signaling pathways. Furthermore, many of the circRNAs were found to interact with miRNAs that are associated with follicular development. This study presents a new perspective for studying circRNAs and provides a valuable resource for further examination into the potential roles of circRNAs in porcine follicular development.
- Subjects :
- 0301 basic medicine
Swine
Biology
Real-Time Polymerase Chain Reaction
lcsh:Gynecology and obstetrics
DNA sequencing
Follicular phase
03 medical and health sciences
0302 clinical medicine
Ovarian Follicle
microRNA
Gene expression
Animals
Sows
KEGG
Gene
lcsh:RG1-991
Meishan
Middle-sized ovarian follicles
Sequence Analysis, RNA
Gene Expression Profiling
Research
Obstetrics and Gynecology
RNA
High-Throughput Nucleotide Sequencing
Molecular Sequence Annotation
RNA, Circular
Cell biology
Reverse transcription polymerase chain reaction
MicroRNAs
030104 developmental biology
Oncology
Gene Expression Regulation
030220 oncology & carcinogenesis
Female
Circular RNAs
Subjects
Details
- ISSN :
- 17572215
- Volume :
- 13
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Journal of ovarian research
- Accession number :
- edsair.doi.dedup.....ff803f965423756bc5026e1323e215d5