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Derivation of induced pluripotent stem cells from ferret somatic cells
- Source :
- Am J Physiol Lung Cell Mol Physiol
- Publication Year :
- 2020
- Publisher :
- American Physiological Society, 2020.
-
Abstract
- Ferrets are an attractive mammalian model for several diseases, especially those affecting the lungs, liver, brain, and kidneys. Many chronic human diseases have been difficult to model in rodents due to differences in size and cellular anatomy. This is particularly the case for the lung, where ferrets provide an attractive mammalian model of both acute and chronic lung diseases, such as influenza, cystic fibrosis, A1A emphysema, and obliterative bronchiolitis, closely recapitulating disease pathogenesis, as it occurs in humans. As such, ferrets have the potential to be a valuable preclinical model for the evaluation of cell-based therapies for lung regeneration and, likely, for other tissues. Induced pluripotent stem cells (iPSCs) provide a great option for provision of enough autologous cells to make patient-specific cell therapies a reality. Unfortunately, they have not been successfully created from ferrets. In this study, we demonstrate the generation of ferret iPSCs that reflect the primed pluripotent state of human iPSCs. Ferret fetal fibroblasts were reprogrammed and acquired core features of pluripotency, having the capacity for self-renewal, multilineage differentiation, and a high-level expression of the core pluripotency genes and pathways at both the transcriptional and protein level. In conclusion, we have generated ferret pluripotent stem cells that provide an opportunity for advancing our capacity to evaluate autologous cell engraftment in ferrets.
- Subjects :
- 0301 basic medicine
Pulmonary and Respiratory Medicine
Male
Physiology
Somatic cell
Cell
Induced Pluripotent Stem Cells
Biology
Cystic fibrosis
03 medical and health sciences
0302 clinical medicine
Physiology (medical)
medicine
Animals
Humans
Induced pluripotent stem cell
Cells, Cultured
Fetus
Lung
Regeneration (biology)
Ferrets
Cell Differentiation
Cell Biology
respiratory system
Fibroblasts
medicine.disease
Cellular Reprogramming
030104 developmental biology
medicine.anatomical_structure
030220 oncology & carcinogenesis
Cancer research
Female
Reprogramming
Research Article
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Am J Physiol Lung Cell Mol Physiol
- Accession number :
- edsair.doi.dedup.....ff6f41ab7b61bd2aeb7c44f28e174476