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Copy number increases of transposable elements and protein-coding genes in an invasive fish of hybrid origin
- Source :
- Molecular Ecology
- Publication Year :
- 2017
-
Abstract
- Evolutionary dynamics of structural genetic variation in lineages of hybrid origin is not well explored, although structural mutations may increase in controlled hybrid crosses. We therefore tested whether structural variants accumulate in a fish of recent hybrid origin, invasive Cottus, relative to both parental species Cottus rhenanus and Cottus perifretum. Copy‐number variation in exons of 10,979 genes was assessed using comparative genome hybridization arrays. Twelve genes showed significantly higher copy numbers in invasive Cottus compared to both parents. This coincided with increased expression for three genes related to vision, detoxification and muscle development, suggesting possible gene dosage effects. Copy number increases of putative transposons were assessed by comparative mapping of genomic DNA reads against a de novo assembly of 1,005 repetitive elements. In contrast to exons, copy number increases of repetitive elements were common (20.7%) in invasive Cottus, whereas decrease was very rare (0.01%). Among the increased repetitive elements, 53.8% occurred at higher numbers in C. perifretum compared to C. rhenanus, while only 1.4% were more abundant in C. rhenanus. This implies a biased mutational process that amplifies genetic material from one ancestor. To assess the frequency of de novo mutations through hybridization, we screened 64 laboratory‐bred F2 offspring between the parental species for copy‐number changes at five candidate loci. We found no evidence for new structural variants, indicating that they are too rare to be detected given our sampling scheme. Instead, they must have accumulated over more generations than we observed in a controlled cross.<br />see also the Perspective by Choudhury and Parisod
- Subjects :
- 0301 basic medicine
DNA Copy Number Variations
hybrid speciation
Cottus perifretum
invasion genetics
03 medical and health sciences
10127 Institute of Evolutionary Biology and Environmental Studies
1311 Genetics
Genetic variation
Genetics
Animals
Speciation and Hybridization
Gene
Ecology, Evolution, Behavior and Systematics
Cottus
array‐comparative genomic hybridization
biology
structural mutations
biology.organism_classification
Cottus rhenanus
Cottidae
Biological Evolution
Perciformes
genomic DNA
030104 developmental biology
1105 Ecology, Evolution, Behavior and Systematics
DNA Transposable Elements
570 Life sciences
590 Animals (Zoology)
Hybridization, Genetic
Hybrid speciation
Original Article
ORIGINAL ARTICLES
Introduced Species
digital droplet PCR
Comparative genomic hybridization
Subjects
Details
- ISSN :
- 1365294X
- Volume :
- 26
- Issue :
- 18
- Database :
- OpenAIRE
- Journal :
- Molecular ecology
- Accession number :
- edsair.doi.dedup.....ff53065d81a324bf1ee6cc7e198a9bac