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Combined positive effect of oocyte extracts and brilliant cresyl blue stained recipient cytoplasts on epigenetic reprogramming and gene expression in buffalo nuclear transfer embryos
- Source :
- Cytotechnology. 69(2)
- Publication Year :
- 2016
-
Abstract
- This study examined the effects of buffalo oocyte extracts (BOE) on donor cells reprogramming and molecular characterisation of oocytes screened via brilliant cresyl blue (BCB) staining and comparison of gene expression profiles of developmentally important genes in blastocysts from IVF and cloned derived from BOE treated donor cells with BCB selected recipient cytoplasts. Relative abundance (RA) of OCT4 and NANOG was increased (P
- Subjects :
- 0301 basic medicine
Homeobox protein NANOG
030219 obstetrics & reproductive medicine
Clinical Biochemistry
Biomedical Engineering
Bioengineering
Embryo
Cell Biology
Biology
Oocyte
Molecular biology
Cytoplast
Embryo transfer
03 medical and health sciences
030104 developmental biology
0302 clinical medicine
medicine.anatomical_structure
SOX2
Gene expression
embryonic structures
medicine
Original Article
Reprogramming
Biotechnology
Subjects
Details
- ISSN :
- 09209069
- Volume :
- 69
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Cytotechnology
- Accession number :
- edsair.doi.dedup.....ff4d2b9fec4ea9d6cf9489b1af31f07a