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Combined positive effect of oocyte extracts and brilliant cresyl blue stained recipient cytoplasts on epigenetic reprogramming and gene expression in buffalo nuclear transfer embryos

Authors :
Kataria Meena
E. M. Sadeesh
Shah Fozia
Source :
Cytotechnology. 69(2)
Publication Year :
2016

Abstract

This study examined the effects of buffalo oocyte extracts (BOE) on donor cells reprogramming and molecular characterisation of oocytes screened via brilliant cresyl blue (BCB) staining and comparison of gene expression profiles of developmentally important genes in blastocysts from IVF and cloned derived from BOE treated donor cells with BCB selected recipient cytoplasts. Relative abundance (RA) of OCT4 and NANOG was increased (P

Details

ISSN :
09209069
Volume :
69
Issue :
2
Database :
OpenAIRE
Journal :
Cytotechnology
Accession number :
edsair.doi.dedup.....ff4d2b9fec4ea9d6cf9489b1af31f07a