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In vitro pharmacoregulation of CC chemokine ligand 5 and its receptor CCR5 in diffuse lung diseases
- Source :
- Mediators of Inflammation, Mediators of Inflammation, Vol 12, Iss 4, Pp 215-220 (2003)
- Publication Year :
- 2003
-
Abstract
- Background:CC chemokine ligand (CCL)5 and its receptor CCR5 contribute to leukocyte migration into lungs of patients with diffuse lung diseases (DLD). Pharmacological regulation of CCL5 and CCR5 expression was therefore explored in bronchoalveolar cells obtained from patients with DLD.Methods:Cells from 21 patients were co-cultivatedin vitrowith tumour necrosis factor-α and dexamethasone, cyclosporin A (CyA) or pentoxifylline. Chemokine mRNA expression and protein production was assessed by reverse transcription-polymerase chain reaction and enzyme-linked immunosorbent assay, respectively.Results:Dexamethasone altered CCL5 mRNA expression and suppressed its protein levels. CyA inhibited chemokine mRNA expression but not protein production. Pentoxifylline did not affected chemokine expression. Both dexamethasone and CyA suppressed CCR5 mRNA transcripts.Conclusion:In conclusion, while dexamethasone downregulates the CCL5 functional form, CyA and pentoxifylline have no effects on CCL5 protein. These data providein vitrocorrelation for clinical applications of immunomodulators in therapy of DLD.
- Subjects :
- Adult
Lung Diseases
Male
CCR2
Leukocyte migration
Pathology
medicine.medical_specialty
Sarcoidosis
Receptors, CCR5
Immunology
Biology
CCL5
Dexamethasone
RANTES
Cyclosporin a
lcsh:Pathology
medicine
Leukocytes
CCL17
Humans
RNA, Messenger
Enzyme Inhibitors
Pentoxifylline
Receptor
Chemokine CCL5
Glucocorticoids
Bronchoalveolar cells
Cells, Cultured
Aged
Lung
Tumor Necrosis Factor-alpha
virus diseases
Cell Biology
respiratory system
Middle Aged
Ligand (biochemistry)
respiratory tract diseases
Cyclosporin A
medicine.anatomical_structure
Chemokines, CC
Cancer research
Cyclosporine
Female
Bronchoalveolar Lavage Fluid
Immunosuppressive Agents
lcsh:RB1-214
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 09629351
- Volume :
- 12
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Mediators of Inflammation
- Accession number :
- edsair.doi.dedup.....ff4a50f76e23a11675959ca1ce3c6694