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In vitro pharmacoregulation of CC chemokine ligand 5 and its receptor CCR5 in diffuse lung diseases

Authors :
Daniela Subrtova
Agata Gibejova
Frantisek Mrazek
Veronika Sekerova
Vitezslav Kolek
Roland M. du Bois
Martin Petrek
Source :
Mediators of Inflammation, Mediators of Inflammation, Vol 12, Iss 4, Pp 215-220 (2003)
Publication Year :
2003

Abstract

Background:CC chemokine ligand (CCL)5 and its receptor CCR5 contribute to leukocyte migration into lungs of patients with diffuse lung diseases (DLD). Pharmacological regulation of CCL5 and CCR5 expression was therefore explored in bronchoalveolar cells obtained from patients with DLD.Methods:Cells from 21 patients were co-cultivatedin vitrowith tumour necrosis factor-α and dexamethasone, cyclosporin A (CyA) or pentoxifylline. Chemokine mRNA expression and protein production was assessed by reverse transcription-polymerase chain reaction and enzyme-linked immunosorbent assay, respectively.Results:Dexamethasone altered CCL5 mRNA expression and suppressed its protein levels. CyA inhibited chemokine mRNA expression but not protein production. Pentoxifylline did not affected chemokine expression. Both dexamethasone and CyA suppressed CCR5 mRNA transcripts.Conclusion:In conclusion, while dexamethasone downregulates the CCL5 functional form, CyA and pentoxifylline have no effects on CCL5 protein. These data providein vitrocorrelation for clinical applications of immunomodulators in therapy of DLD.

Details

Language :
English
ISSN :
09629351
Volume :
12
Issue :
4
Database :
OpenAIRE
Journal :
Mediators of Inflammation
Accession number :
edsair.doi.dedup.....ff4a50f76e23a11675959ca1ce3c6694