Back to Search
Start Over
A thermophile under pressure: Transcriptional analysis of the response of Caldicellulosiruptor saccharolyticus to different H2 partial pressures
- Source :
- International Journal of Hydrogen Energy 38 (2013) 4, International Journal of Hydrogen Energy, 38(4), 1837-1849
- Publication Year :
- 2013
-
Abstract
- Increased hydrogen (H2) levels are known to inhibit H2 formation in Caldicellulosiruptor saccharolyticus. To investigate this organism's strategy for dealing with elevated H2 levels the effect of the hydrogen partial pressure ( P H 2 ) on fermentation performance was studied by growing cultures under high and low P H 2 in a glucose limited chemostat setup. Transcriptome analysis revealed the upregulation of genes involved in the disposal of reducing equivalents under high P H 2 , like lactate dehydrogenase and alcohol dehydrogenase as well as the NADH-dependent and ferredoxin-dependent hydrogenases. These findings are in line with the observed shift in fermentation profiles from acetate production to the production of acetate, lactate and ethanol under high P H 2 . Moreover, differential transcription was observed for genes involved in carbon metabolism, fatty acid biosynthesis and several transport systems. In addition, presented transcription data provide evidence for the involvement of the redox sensing Rex protein in gene regulation under high P H 2 cultivation conditions.
- Subjects :
- Energy Engineering and Power Technology
Chemostat
extreme thermophiles
Microbiology
thermotoga-neapolitana
chemistry.chemical_compound
ferredoxin oxidoreductase
Microbiologie
Lactate dehydrogenase
Alcohol dehydrogenase
VLAG
cellulolytic bacterium
alcohol dehydrogenases
Ethanol
WIMEK
biology
Renewable Energy, Sustainability and the Environment
Thermophile
hyperthermophilic archaeon
rex-family repressor
Condensed Matter Physics
biology.organism_classification
hydrogen-production
Fuel Technology
thermoanaerobacter-ethanolicus
chemistry
Biochemistry
biology.protein
Fermentation
pyrococcus-furiosus
Caldicellulosiruptor saccharolyticus
Thermotoga neapolitana
Subjects
Details
- Language :
- English
- ISSN :
- 03603199
- Volume :
- 38
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- International Journal of Hydrogen Energy
- Accession number :
- edsair.doi.dedup.....ff3c6818891aa4cd8c99ff10a341d792