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Genetic analysis and gene fine mapping of aroma in rice (Oryza sativa L. Cyperales, Poaceae)
- Source :
- Genetics and Molecular Biology, Vol 31, Iss 2, Pp 532-538 (2008), Genetics and Molecular Biology, Volume: 31, Issue: 2, Pages: 532-538, Published: 2008, Genetics and Molecular Biology v.31 n.2 2008, Genetics and Molecular Biology, Sociedade Brasileira de Genética (SBG), instacron:SBG
- Publication Year :
- 2008
- Publisher :
- FapUNIFESP (SciELO), 2008.
-
Abstract
- We investigated inheritance and carried out gene fine mapping of aroma in crosses between the aromatic elite hybrid rice Oryza sativa indica variety Chuanxiang-29B (Ch-29B) and the non-aromatic rice O. sativa indica variety R2 and O. sativa japonica Lemont (Le). The F1 grains and leaves were non-aromatic while the F2 non-aroma to aroma segregation pattern was 3:1. The F3 segregation ratio was consistent with the expected 1:2:1 for a single recessive aroma gene in Ch-29B. Linkage analysis between simple sequence repeat (SSR) markers and the aroma locus for the aromatic F2 plants mapped the Ch-29B aroma gene to a chromosome 8 region flanked by SSR markers RM23120 at 0.52 cM and RM3459 at 1.23 cM, a replicate F2 population confirming these results. Three bacterial artificial chromosome (BAC) clones cover chromosome 8 markers RM23120 and RM3459. Our molecular mapping data from the two populations indicated that the aroma locus occurs in a 142.85 kb interval on BAC clones AP005301 or AP005537, implying that it might be the same gene reported by Bradbury et al (2005a; Plant Biotec J. 3:363-370). The flanking markers Aro7, RM23120 and RM3459 identified by us could greatly accelerate the efficiency and precision of aromatic rice breeding programs.
- Subjects :
- education.field_of_study
Oryza sativa
lcsh:QH426-470
Population
food and beverages
Locus (genetics)
genetic analysis
Biology
biology.organism_classification
Genetic analysis
lcsh:Genetics
aroma
Genetic linkage
Botany
Genetics
gene fine mapping
Poaceae
rice (Oryza sativa L.)
education
Molecular Biology
Gene
Aroma
Subjects
Details
- ISSN :
- 14154757
- Volume :
- 31
- Database :
- OpenAIRE
- Journal :
- Genetics and Molecular Biology
- Accession number :
- edsair.doi.dedup.....ff2b7fc97ec4104b40a77a6edbe26e07