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Establishment and characterization of a mouse embryonic heart slice preparation
- Source :
- Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology. 16(1-3)
- Publication Year :
- 2005
-
Abstract
- Background: In contrast to isolated cells, the anatomic and functional integrity of tissue slices remains preserved. Aim of the study was to establish the slice technique in embryonic mouse hearts in order to perform physiological and pharmacological investigations of wild-type mice and genetically engineered mouse models of heart disease. Methods: Ventricular slices (thickness: 300 µm) were cut from agar-embedded embryonic mouse hearts (ED 16.5-18.5) with a vibratome. Histology, immunostaining with markers for apoptosis induction, intracellular recordings with sharp electrodes and field potential recordings using microelectrode arrays were performed to assess viability. Results: Slices exhibited normal histology without prominent signs of apoptosis for at least 24 hours. Intracellular recordings revealed the typical electrophysiological fingerprint of ventricular cardiomyocytes. Field potential recordings proved that adrenergic and muscarinic signaling was preserved. Conclusion: Functionally intact heart slices can be generated from murine embryos.
- Subjects :
- Pathology
medicine.medical_specialty
Embryonic heart
Staining and Labeling
Physiology
Isoproterenol
Action Potentials
Apoptosis
Biology
Electrophysiology
Tissue Culture Techniques
Vibratome
Mice
Slice preparation
Fetal Heart
Heart Rate
Genetically Engineered Mouse
Muscarinic acetylcholine receptor
medicine
Animals
Carbachol
Intracellular
Immunostaining
Subjects
Details
- ISSN :
- 10158987
- Volume :
- 16
- Issue :
- 1-3
- Database :
- OpenAIRE
- Journal :
- Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology
- Accession number :
- edsair.doi.dedup.....ff02c090ea3b36040d381082d7fe6471