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Interaction between atrial natriuretic peptide and vasoactive intestinal peptide in guinea pig cecal smooth muscle
- Source :
- Gastroenterology. 109(4)
- Publication Year :
- 1995
-
Abstract
- Backgrounds & Aims: The role of atrial natriuretic peptide (ANP) in gastrointestinal motility is still unclear. The aim of this study was to investigate the relationship between ANP and vasoactive intestinal peptide (VIP) in guinea pig cecal circular smooth muscle cells. Methods: The inhibition of 125 I-ANP binding or 125 I-VIP binding to cecal smooth muscle cells was assessed using unlabeled peptides (i.e., ANP, ANP fragments, VIP, secretin, and peptide histidine isoleucine); the effect of ANP, ANP fragments, and VIP on muscle contraction stimulated by 1 μmol/L carbachol was assessed; and the inhibitory effects of ANP 1–11 on VIP-induced relaxation, ANP 1–11 and VIP 10–28 (a VIP antagonist) on ANP-induced relaxation, and nitric oxide production inhibitors on ANP-induced relaxation were assessed. Results: The specific binding of 125 I-ANP was inhibited completely by unlabeled ANP and VIP in a dose-dependent manner but only slightly inhibited by secretin and peptide histidine isoleucine. ANP 1–11 and C-atrial natriuretic factor inhibited the binding of 125 I-ANP with a lower affinity than ANP. ANP only partly inhibited 125 I-VIP binding. ANP and VIP inhibited 1 μmol/L carbachol-induced contraction in a dose-dependent manner. ANP 1–11 significantly inhibited VIP-induced relaxation. ANP 1–11, VIP 10–28, and NO production inhibitors completely inhibited ANP-induced relaxation. Conclusions: The results of the study showed that ANP 1–11 antagonized ANP-induced relaxation and that ANP stimulated NO production and subsequently induced relaxation via a receptor to which VIP binds.
- Subjects :
- Male
medicine.medical_specialty
Carbachol
Vasoactive intestinal peptide
Guinea Pigs
Nitric Oxide
Secretin
Nitric oxide
Guinea pig
chemistry.chemical_compound
Atrial natriuretic peptide
Internal medicine
medicine
Animals
Receptor
Cecum
Cells, Cultured
Hepatology
Gastroenterology
Muscle, Smooth
Endocrinology
chemistry
cardiovascular system
medicine.symptom
Gastrointestinal Motility
hormones, hormone substitutes, and hormone antagonists
Atrial Natriuretic Factor
circulatory and respiratory physiology
medicine.drug
Muscle contraction
Muscle Contraction
Vasoactive Intestinal Peptide
Subjects
Details
- ISSN :
- 00165085
- Volume :
- 109
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Gastroenterology
- Accession number :
- edsair.doi.dedup.....fefb755c0124f9baafece60dcb07420f