Back to Search Start Over

Plasmacytoid DCs regulate recall responses by rapid induction of IL-10 in memory T cells

Authors :
Fridtjof Lund-Johansen
Per Brandtzaeg
Johanna Olweus
Yngvar Fløisand
Smita Ghanekar
Frode L. Jahnsen
Halvor Rollag
Espen O. Kvale
Lorant Farkas
Source :
Blood. 109(8)
Publication Year :
2006

Abstract

Dendritic cells (DCs) are believed to regulate T cell-mediated immunity primarily by directing differentiation of naive T cells. Here, we show that a large fraction of CD4+ memory cells produce IL-10 within the first hours after interaction with plasmacytoid DCs (PDCs). In contrast, CD11c+ DCs induce IFN-γ and little IL-10. IL-10–secreting T cells isolated after 36 hours of culture with PDCs suppressed antigen-induced T-cell proliferation by an IL-10–dependent mechanism, but were distinct from natural and type 1 regulatory T cells. They proliferated strongly and continued to secrete IL-10 during expansion with PDCs, and after restimulation with immature monocyte-derived DCs or CD11c+ DCs. The IL-10–producing T cells acquired the ability to secrete high levels of IFN-γ after isolation and subsequent coculture with PDCs or CD11c+ DCs. Compared to CD11c+ DCs, PDCs were superior in their ability to selectively expand T cells that produced cytokines on repeated antigenic challenge. The DC-dependent differences in cytokine profiles were observed with viral recall antigen or staphylococcal enterotoxin B and were independent of extracellular type I interferon or IL-10. Our results show that DCs can regulate memory responses and that PDCs rapidly induce regulatory cytokines in effector T cells that can suppress bystander activity.

Details

ISSN :
00064971
Volume :
109
Issue :
8
Database :
OpenAIRE
Journal :
Blood
Accession number :
edsair.doi.dedup.....fefa7ee1b9ee25c7df33e3d926ada46e