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Plasmacytoid DCs regulate recall responses by rapid induction of IL-10 in memory T cells
- Source :
- Blood. 109(8)
- Publication Year :
- 2006
-
Abstract
- Dendritic cells (DCs) are believed to regulate T cell-mediated immunity primarily by directing differentiation of naive T cells. Here, we show that a large fraction of CD4+ memory cells produce IL-10 within the first hours after interaction with plasmacytoid DCs (PDCs). In contrast, CD11c+ DCs induce IFN-γ and little IL-10. IL-10–secreting T cells isolated after 36 hours of culture with PDCs suppressed antigen-induced T-cell proliferation by an IL-10–dependent mechanism, but were distinct from natural and type 1 regulatory T cells. They proliferated strongly and continued to secrete IL-10 during expansion with PDCs, and after restimulation with immature monocyte-derived DCs or CD11c+ DCs. The IL-10–producing T cells acquired the ability to secrete high levels of IFN-γ after isolation and subsequent coculture with PDCs or CD11c+ DCs. Compared to CD11c+ DCs, PDCs were superior in their ability to selectively expand T cells that produced cytokines on repeated antigenic challenge. The DC-dependent differences in cytokine profiles were observed with viral recall antigen or staphylococcal enterotoxin B and were independent of extracellular type I interferon or IL-10. Our results show that DCs can regulate memory responses and that PDCs rapidly induce regulatory cytokines in effector T cells that can suppress bystander activity.
- Subjects :
- medicine.medical_treatment
Immunology
Plasma Cells
CD11c
chemical and pharmacologic phenomena
Biology
Biochemistry
T-Lymphocytes, Regulatory
Enterotoxins
Interferon-gamma
Antigen
Interferon
Immunity
medicine
Humans
Secretion
Antigens, Viral
Cells, Cultured
Effector
hemic and immune systems
Cell Biology
Hematology
Bystander Effect
Dendritic Cells
Coculture Techniques
CD11c Antigen
Interleukin-10
Interleukin 10
Cytokine
Interferon Type I
Immunologic Memory
medicine.drug
Subjects
Details
- ISSN :
- 00064971
- Volume :
- 109
- Issue :
- 8
- Database :
- OpenAIRE
- Journal :
- Blood
- Accession number :
- edsair.doi.dedup.....fefa7ee1b9ee25c7df33e3d926ada46e