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Comparison of diagnostic potential of serological, molecular and cell culture methods for detection of Q fever in ruminants
- Source :
- Veterinary Microbiology
-
Abstract
- Q fever is an infectious disease caused by Coxiella burnetii. Diagnosis of Q fever based on clinical symptoms is unattainable; thus, different laboratory techniques are used to detect the infection. The aim of the study was to compare the diagnostic potential of ELISA, CFT, conventional PCR, real-time PCR and cell culture. The tests were carried out on 2251 serum samples from ruminants. Moreover, 668 placentas, 1277 vaginal swabs and 306 specimens of the internal organs of aborted foetuses were examined by PCR and cell culture. Pearson's chi-square test was used to compare the results obtained by ELISA, CFT, PCR, real-time PCR and isolation in cell culture. The χ(2) test confirmed that in most cases the results obtained by means of the different methods were correlated with each other (P
- Subjects :
- 040301 veterinary sciences
Placenta
Cell Culture Techniques
Cattle Diseases
Sheep Diseases
Q fever
Enzyme-Linked Immunosorbent Assay
Microbiology
Polymerase Chain Reaction
law.invention
Serology
0403 veterinary science
03 medical and health sciences
law
Pregnancy
Chlorocebus aethiops
medicine
Animals
Vero Cells
Polymerase chain reaction
0303 health sciences
Laboratory methods
Goat Diseases
Sheep
General Veterinary
biology
030306 microbiology
Goats
Reproducibility of Results
04 agricultural and veterinary sciences
General Medicine
Ruminants
Coxiella burnetii
biology.organism_classification
medicine.disease
Serum samples
Virology
Molecular biology
3. Good health
Cell culture
Vaginal swabs
Cattle
Female
Q Fever
Subjects
Details
- Language :
- English
- ISSN :
- 03781135
- Volume :
- 171
- Issue :
- 1-2
- Database :
- OpenAIRE
- Journal :
- Veterinary Microbiology
- Accession number :
- edsair.doi.dedup.....fef693ca3094fdcc589460c3ae40e287
- Full Text :
- https://doi.org/10.1016/j.vetmic.2014.03.015