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Psychological stress increases human T cell apoptosis in vitro
- Source :
- Neuroimmunomodulation. 10(4)
- Publication Year :
- 2002
-
Abstract
- Objectives: Recent studies have shown that apoptosis is involved in stress responses. The present study examined if stressors increase in vitro apoptosis of peripheral blood T lymphocytes in a dose-dependent manner. Methods: Daily subjective stress was quantitatively analyzed in 40 nonsmoking men with a daily hassles questionnaire. Apoptosis of T lymphocytes was measured by flowcytometry using Annexin V/PI double staining method after 0, 12, and 24 h of culture in the presence or absence of dexamethasone (DEX). Using a cross-sectional design, the current study examined the relationship between stress and in vitro apoptosis of T cells. Results: Results showed that apoptosis of T lymphocytes in vitro has a significant correlation with stress and age. Stress was positively correlated with percentage of apoptosis in T cells after 12 h of culture, irrespective of DEX treatment. Age was positively correlated with the percentage of T cell apoptosis after 0 and 12 h of coculture with DEX. Conclusions: These results indicate that age-related apoptosis and stress-related apoptosis of T cells are modulated through different mechanisms. This is the first study to show that in vitro lymphocyte apoptosis is influenced by daily stress in a dose-dependent manner.
- Subjects :
- Male
Aging
Neuroimmunomodulation
T-Lymphocytes
Immunology
Apoptosis
Phosphatidylserines
medicine.disease_cause
Dexamethasone
Flow cytometry
Endocrinology
Surveys and Questionnaires
Activities of Daily Living
medicine
Reaction Time
Psychological stress
Humans
Annexin A5
Cells, Cultured
T-cell apoptosis
medicine.diagnostic_test
Endocrine and Autonomic Systems
business.industry
Flow Cytometry
Immunohistochemistry
In vitro
Neuroimmunology
Neurology
Cancer research
Lymph
business
Stress, Psychological
Subjects
Details
- ISSN :
- 10217401
- Volume :
- 10
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Neuroimmunomodulation
- Accession number :
- edsair.doi.dedup.....fef5799c0829ff41173ba9f3e6a072a3