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Position-dependent expression of GADD45alpha in rat brain tumours
- Source :
- Medical oncology (Northwood, London, England). 24(4)
- Publication Year :
- 2007
-
Abstract
- Although the complex and multifactorial process of tumour growth has been extensively studied for decades, our understanding of the fundamental relationship between tumour growth dynamics and genetic expression profile remains incomplete. Recent studies of tumour dynamics indicate that gene expression in solid tumours would depend on the distance from the centre of the tumour. Since tumour proliferative activity is mainly localised to its external zone, and taking into account that generation and expansion of genetic mutations depend on the number of cell divisions, important differences in gene expression between central and peripheral sections of the same tumour are to be expected. Here, we have studied variations in the genetic expression profile between peripheral and internal samples of the same brain tumour. We have carried out microarray analysis of mRNA expression, and found a differential profile of genetic expression between the two cell subsets. In particular, one major nuclear protein that regulates cell responses to DNA-damaging and stress signals, GADD45alpha, was expressed at much lower levels in the peripheral zone, as compared to tumour core samples. These differences in GADD45alpha mRNA transcription levels have been confirmed by quantitative analysis via real time PCR, and protein levels of GADD45alpha also exhibit the same pattern of differential expression. Our findings suggest that GADD45alpha might play a major role in the regulation of brain tumour invasive potential.
- Subjects :
- Cyclin-Dependent Kinase Inhibitor p21
Cancer Research
medicine.medical_specialty
Transcription, Genetic
Cell
Cell Cycle Proteins
Biology
Transcription (biology)
Internal medicine
Gene expression
medicine
Animals
Neoplasm Invasiveness
RNA, Messenger
Nuclear protein
Rats, Wistar
Cell Proliferation
Oligonucleotide Array Sequence Analysis
Messenger RNA
Hematology
Microarray analysis techniques
Brain Neoplasms
Gene Expression Profiling
Nuclear Proteins
General Medicine
Molecular biology
Magnetic Resonance Imaging
Rats
Real-time polymerase chain reaction
medicine.anatomical_structure
Oncology
Cancer research
Subjects
Details
- ISSN :
- 13570560
- Volume :
- 24
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Medical oncology (Northwood, London, England)
- Accession number :
- edsair.doi.dedup.....feefae0341d5bb2ca59df47c67279e38