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Construction and evaluation of DNA vaccine encoding Ebola virus glycoprotein fused with lysosome-associated membrane protein
- Source :
- Antiviral research. 193
- Publication Year :
- 2021
-
Abstract
- Ebola virus (EBOV) of the genus Ebolavirus belongs to the family Filoviridae, which cause disease in both humans and non-human primates. Zaire Ebola virus accounts for the highest fatality rate, reaching 90%. Considering that EBOV has a high infection and fatality rate, the development of a highly effective vaccine has become a top public health priority. Glycoprotein (GP) plays a critical role during infection and protective immune responses. Herein, we developed an EBOV GP recombinant DNA vaccine that targets the major histocompatibility complex (MHC) class II compartment by fusing with lysosomal-associated membrane protein 1 (LAMP1). Through lysosome trafficking and antigen presentation transferring, the LAMP1 targeting strategy successfully improved both humoral and cellular EBOV-GP-specific immune responses. After three consecutive immunizations, the serum antibody titers, especially the neutralizing activity of mice immunized with the pVAX-LAMP/GPEBO vaccine were significantly higher than those of the other groups. Antigen-specific T cells showed positive activity against three dominant peptides, EAAVSHLTTLATIST, IGEWAFWETKKNLTR, and ELRTFSILNRKAIDF, with high affinity for MHC class II molecules predicted by IEDB-recommended. Preliminary safety observation denied histological alterations. DNA vaccine candidate pVAX-LAMP/GPEBO shows promise against Ebola epidemic and further evaluation is guaranteed.
- Subjects :
- BALB 3T3 Cells
Recombinant Fusion Proteins
Antigen presentation
Filoviridae
medicine.disease_cause
Major histocompatibility complex
Antibodies, Viral
law.invention
DNA vaccination
Mice
Immune system
law
Neutralization Tests
Virology
medicine
Vaccines, DNA
Animals
Humans
Ebola Vaccines
Glycoproteins
Pharmacology
MHC class II
Vaccines, Synthetic
Ebola virus
biology
Lysosome-Associated Membrane Glycoproteins
Hemorrhagic Fever, Ebola
biology.organism_classification
Ebolavirus
Antibodies, Neutralizing
biology.protein
Recombinant DNA
Female
Subjects
Details
- ISSN :
- 18729096
- Volume :
- 193
- Database :
- OpenAIRE
- Journal :
- Antiviral research
- Accession number :
- edsair.doi.dedup.....fecd4a95d4f9384bc95bcdc65cf013e1