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The effect of lidocaine on TRPM 2,6,7 and 8 channels in liver ischemia / reperfusion model in rats
- Source :
- Annals of Clinical and Analytical Medicine. 11
- Publication Year :
- 2020
- Publisher :
- Journal of Clinical and Analytical Medicine, 2020.
-
Abstract
- Aim: The transient receptor potential melastatin (TRPM) channel is subfamilies of the transient receptor potential (TRP) channels, cation permeable channels. Ischemia and reperfusion (I / R) injury is a phenomenon highlighting cellular damage of the liver caused by oxygen. Lidocaine is a local anesthetic that blocks sodium channels and suppresses mitochondrial functions of neutrophils. In this study, we purpose to investigate the effects of TRPM2/8 and TRPM6/7 expressions after lidocaine treatment in liver ischemia/reperfusion rat model. Materials and Methods: The study was carried out on 32 male Wistar rats. The animals were randomly divided into 4 groups including sham, lidocaine, I/R group and I/R- lidocaine group. The portal vein and the hepatic artery branches were clamped for 60 minutes for complete ischemia. TRPM2/8 and TRPM6/7 gene expression levels were assessed by RT-qPCR. Results: The expression levels of TRPM2 were significantly higher in liver-I/R group compared to sham and lidocaine groups (p
Details
- ISSN :
- 2667663X
- Volume :
- 11
- Database :
- OpenAIRE
- Journal :
- Annals of Clinical and Analytical Medicine
- Accession number :
- edsair.doi.dedup.....fec6240e13383dd1be05719d06e6854e