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The Time-Feature of Uric Acid Excretion in Hyperuricemia Mice Induced by Potassium Oxonate and Adenine
- Source :
- International Journal of Molecular Sciences, Volume 21, Issue 15, International Journal of Molecular Sciences, Vol 21, Iss 5178, p 5178 (2020)
- Publication Year :
- 2020
- Publisher :
- Multidisciplinary Digital Publishing Institute, 2020.
-
Abstract
- Hyperuricemia is an important risk factor of chronic kidney disease, metabolic syndrome and cardiovascular disease. We aimed to assess the time-feature relationship of hyperuricemia mouse model on uric acid excretion and renal function. A hyperuricemia mouse model was established by potassium oxonate (PO) and adenine for 21 days. Ultra Performance Liquid Chromatography was used to determine plasma uric acid level. Hematoxylin-eosin staining was applied to observe kidney pathological changes, and Western blot was used to detect renal urate transporters&rsquo<br />expression. In hyperuricemia mice, plasma uric acid level increased significantly from the 3rd day, and tended to be stable from the 7th day, and the clearance rate of uric acid decreased greatly from the 3rd day. Further study found that the renal organ of hyperuricemia mice showed slight damage from the 3rd day, and significantly deteriorated renal function from the 10th day. In addition, the expression levels of GLUT9 and URAT1 were upregulated from the 3rd day, while ABCG2 and OAT1 were downregulated from the 3rd day, and NPT1 were downregulated from the 7th day in hyperuricemia mice kidney. This paper presents a method suitable for experimental hyperuricemia mouse model, and shows the time-feature of each index in a hyperuricemia mice model.
- Subjects :
- Time Factors
Glucose Transport Proteins, Facilitative
Organic Anion Transporters
hyperuricemia
Kidney
urologic and male genital diseases
lcsh:Chemistry
chemistry.chemical_compound
Mice
urate transporter
ATP Binding Cassette Transporter, Subfamily G, Member 2
Hyperuricemia
lcsh:QH301-705.5
Spectroscopy
Chromatography, High Pressure Liquid
medicine.diagnostic_test
uric acid excretion
General Medicine
Computer Science Applications
medicine.anatomical_structure
Creatinine
Sodium-Phosphate Cotransporter Proteins, Type I
medicine.medical_specialty
renal injury
mouse model
Renal function
Catalysis
Article
Inorganic Chemistry
Organic Anion Transport Protein 1
Western blot
Internal medicine
medicine
Animals
Physical and Theoretical Chemistry
Molecular Biology
business.industry
Adenine
Organic Chemistry
nutritional and metabolic diseases
medicine.disease
Staining
Uric Acid
Disease Models, Animal
Oxonic Acid
Endocrinology
chemistry
lcsh:Biology (General)
lcsh:QD1-999
Uric acid
Metabolic syndrome
business
Kidney disease
Subjects
Details
- Language :
- English
- ISSN :
- 14220067
- Database :
- OpenAIRE
- Journal :
- International Journal of Molecular Sciences
- Accession number :
- edsair.doi.dedup.....feb4db2e3fcf33b1cd3d3b8a4ef367e0
- Full Text :
- https://doi.org/10.3390/ijms21155178