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Delayed Intervention With Pyridoxamine Improves Metabolic Function and Prevents Adipose Tissue Inflammation and Insulin Resistance in High-Fat Diet–Induced Obese Mice
- Source :
- Diabetes, 65(4), 956-966. American Diabetes Association
- Publication Year :
- 2015
- Publisher :
- American Diabetes Association, 2015.
-
Abstract
- Obesity is associated with an increased risk for the development of type 2 diabetes and vascular complications. Advanced glycation end products are increased in adipose tissue and have been associated with insulin resistance, vascular dysfunction, and inflammation of adipose tissue. Here, we report that delayed intervention with pyridoxamine (PM), a vitamin B6 analog that has been identified as an antiglycating agent, protected against high-fat diet (HFD)–induced body weight gain, hyperglycemia, and hypercholesterolemia, compared with mice that were not treated. In both HFD-induced and db/db obese mice, impaired glucose metabolism and insulin resistance were prevented by PM supplementation. PM inhibited the expansion of adipose tissue and adipocyte hypertrophy in mice. In addition, adipogenesis of murine 3T3-L1 and human Simpson-Golabi-Behmel Syndrome preadipocytes was dose- and time-dependently reduced by PM, as demonstrated by Oil Red O staining and reduced expression of adipogenic differentiation genes. No ectopic fat deposition was found in the liver of HFD mice. The high expression of proinflammatory genes in visceral adipose tissue of the HFD group was significantly attenuated by PM. Treatment with PM partially prevented HFD-induced mild vascular dysfunction. Altogether, these findings highlight the potential of PM to serve as an intervention strategy in obesity.
- Subjects :
- Heart Defects, Congenital
Male
0301 basic medicine
medicine.medical_specialty
Panniculitis
Endocrinology, Diabetes and Metabolism
Mice, Obese
Adipose tissue
030209 endocrinology & metabolism
Inflammation
Type 2 diabetes
Biology
Carbohydrate metabolism
Diet, High-Fat
Drug Administration Schedule
Gigantism
Time-to-Treatment
Proinflammatory cytokine
Mice
03 medical and health sciences
0302 clinical medicine
Insulin resistance
3T3-L1 Cells
Intellectual Disability
Internal medicine
Internal Medicine
medicine
Animals
Humans
Obesity
Cells, Cultured
Arrhythmias, Cardiac
Genetic Diseases, X-Linked
medicine.disease
Mice, Inbred C57BL
030104 developmental biology
Endocrinology
Adipose Tissue
Adipogenesis
Insulin Resistance
Adipocyte hypertrophy
medicine.symptom
Pyridoxamine
Subjects
Details
- ISSN :
- 1939327X and 00121797
- Volume :
- 65
- Database :
- OpenAIRE
- Journal :
- Diabetes
- Accession number :
- edsair.doi.dedup.....fea215cb1573086c7a12daf254d0f18a
- Full Text :
- https://doi.org/10.2337/db15-1390