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Genetic variants associated with the progression of hepatocellular carcinoma in hepatitis C Egyptian patients
- Source :
- Gene. 527(2)
- Publication Year :
- 2013
-
Abstract
- Background Hepatocellular carcinoma (HCC) associated to infection with hepatitis C virus (HCV) has become the fastest-rising cause of cancer-related deaths. Genetic variations may play an important role in the development of HCC in HCV patients. Ghrelin exerts anti-inflammatory, antifibrotic and hepatoprotective effects on chronically injured hepatic tissues. Ghrelin gene shows several single nucleotide polymorphisms (SNPs) including − 604G/A, Arg51Gln, and Leu72Met. Hemochromatosis gene (HFE) mutations namely C282Y and H63D may cause hepatic iron overload, thus increasing the risk of HCC in HCV patients. Aim To investigate the association of progression of HCC with ghrelin and HFE gene polymorphisms in HCV Egyptian patients. Methods Seventy-nine chronic HCV patients (thirty-nine developed HCC and forty did not), and forty healthy control subjects were included in the study. The polymorphisms were evaluated by PCR/RFLP analysis, and related protein levels were measured by either ELISA or colorimetric assays. Results The three tested SNPs on ghrelin gene were detected in the studied groups, only one SNP (Arg51Gln) showed significantly higher GA, AA genotypes and A allele frequencies in hepatitis C patients who developed HCC than in hepatitis C patients without HCC and controls. Of the two mutations studied on HFE gene only H63D heterozygous allele was detected, and its frequency did not statistically differ among studied groups. Conclusion Our results suggest that A allele at position 346 of the ghrelin gene is associated with susceptibility to HCC in hepatitis C patients.
- Subjects :
- Adult
Male
medicine.medical_specialty
Carcinoma, Hepatocellular
Hepatitis C virus
Single-nucleotide polymorphism
Biology
medicine.disease_cause
Gastroenterology
Polymerase Chain Reaction
Internal medicine
Genetics
medicine
Humans
Allele
Allele frequency
DNA Primers
Base Sequence
Liver Neoplasms
Genetic Variation
General Medicine
Hepatitis C
Middle Aged
medicine.disease
digestive system diseases
Hepatocellular carcinoma
Hereditary hemochromatosis
Case-Control Studies
Cancer research
Disease Progression
Ghrelin
Egypt
Female
Subjects
Details
- ISSN :
- 18790038
- Volume :
- 527
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Gene
- Accession number :
- edsair.doi.dedup.....fe973aab0e7a9a87a24cdf1d3e995047