Back to Search
Start Over
Baeyer-Villiger oxidation of progesterone by Aspergillus sojae PTCC 5196
- Source :
- Steroids. 140:52-57
- Publication Year :
- 2018
- Publisher :
- Elsevier BV, 2018.
-
Abstract
- Microbial transformations are capable of producing steroid substances difficult to synthesize by chemical methods. Strains belonging to the genus Aspergillus are effective facilitators of microbial biotransformations due to their enzymatic diversity. In this study, the biotransformation of progesterone by the fungus Aspergillus sojae (A. sojae) PTCC 5196 was examined. Analysis of the bioconversion process revealed that progesterone was converted to testololactone through a three-step pathway (17β-acetyl side chain cleavage, 17β-hydroxyl oxidation, and oxygenative lactonization of 17-ketone), indicating the presence of Baeyer-Villiger monooxygenase (BVMO) activity in the fungal strain. GC analysis confirmed the production of testololactone with a yield of 99% in 24 h. Faster testololactone production was induced in the presence of both C-21 (progesterone) and C-19 (androstenedione, testosterone, and dehydroepiandrosterone [DHEA]) steroid substances. Due to the high biotransformation rate observed in the present study, A. sojae may be a novel and promising candidate in the production of testololactone.
- Subjects :
- Bioconversion
medicine.medical_treatment
Clinical Biochemistry
Dehydroepiandrosterone
Aspergillus sojae
010402 general chemistry
01 natural sciences
Biochemistry
Steroid
Endocrinology
Biotransformation
medicine
Molecular Biology
Progesterone
Pharmacology
Aspergillus
biology
010405 organic chemistry
Chemistry
Organic Chemistry
Monooxygenase
biology.organism_classification
0104 chemical sciences
Baeyer–Villiger oxidation
Oxidation-Reduction
Subjects
Details
- ISSN :
- 0039128X
- Volume :
- 140
- Database :
- OpenAIRE
- Journal :
- Steroids
- Accession number :
- edsair.doi.dedup.....fe960daa9cacbf255ab50858376cbf19