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Functional Redundancy of Cyclase-Associated Proteins CAP1 and CAP2 in Differentiating Neurons
- Source :
- Cells, Volume 10, Issue 6, Cells, Vol 10, Iss 1525, p 1525 (2021)
- Publication Year :
- 2021
-
Abstract
- Cyclase-associated proteins (CAPs) are evolutionary-conserved actin-binding proteins with crucial functions in regulating actin dynamics, the spatiotemporally controlled assembly and disassembly of actin filaments (F-actin). Mammals possess two family members (CAP1 and CAP2) with different expression patterns. Unlike most other tissues, both CAPs are expressed in the brain and present in hippocampal neurons. We recently reported crucial roles for CAP1 in growth cone function, neuron differentiation, and neuron connectivity in the mouse brain. Instead, CAP2 controls dendritic spine morphology and synaptic plasticity, and its dysregulation contributes to Alzheimer’s disease pathology. These findings are in line with a model in which CAP1 controls important aspects during neuron differentiation, while CAP2 is relevant in differentiated neurons. We here report CAP2 expression during neuron differentiation and its enrichment in growth cones. We therefore hypothesized that CAP2 is relevant not only in excitatory synapses, but also in differentiating neurons. However, CAP2 inactivation neither impaired growth cone morphology and motility nor neuron differentiation. Moreover, CAP2 mutant mice did not display any obvious changes in brain anatomy. Hence, differently from CAP1, CAP2 was dispensable for neuron differentiation and brain development. Interestingly, overexpression of CAP2 rescued not only growth cone size in CAP1-deficient neurons, but also their morphology and differentiation. Our data provide evidence for functional redundancy of CAP1 and CAP2 in differentiating neurons, and they suggest compensatory mechanisms in single mutant neurons.
- Subjects :
- 0301 basic medicine
Dendritic spine
QH301-705.5
Neurogenesis
Motility
Hippocampal formation
Biology
Article
actin dynamics
03 medical and health sciences
cyclase-associated protein
F-actin
Mice
0302 clinical medicine
medicine
Animals
Biology (General)
Growth cone
Actin
Neurons
Cell Differentiation
General Medicine
Cell biology
growth cone
030104 developmental biology
medicine.anatomical_structure
nervous system
Synaptic plasticity
Neuron differentiation
CAP2
CAP1
Neuron
SRV2
Carrier Proteins
030217 neurology & neurosurgery
Subjects
Details
- ISSN :
- 20734409
- Volume :
- 10
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- Cells
- Accession number :
- edsair.doi.dedup.....fe90dc0cfe9ff8ebd6da0b63522de3cb