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Bacterial host and reporter gene optimization for genetically encoded whole cell biosensors
- Source :
- Environmental Science and Pollution Research, Environmental Science and Pollution Research, Springer Verlag, 2017, 24 (1), pp.52--65. ⟨10.1007/s11356-016-6952-2⟩, Environmental Science and Pollution Research, Springer Verlag, 2017, 24 (1), pp.52--65. 〈10.1007/s11356-016-6952-2〉, Environmental Science and Pollution Research, 2017, 24 (1), pp.52--65. ⟨10.1007/s11356-016-6952-2⟩
- Publication Year :
- 2017
- Publisher :
- HAL CCSD, 2017.
-
Abstract
- International audience; Whole-cell biosensors based on reporter genes allow detection of toxic metals in water with high selectivity and sensitivity under laboratory conditions; nevertheless, their transfer to a commercial inline water analyzer requires specific adaptation and optimization to field conditions as well as economical considerations. We focused here on both the influence of the bacterial host and the choice of the reporter gene by following the responses of global toxicity biosensors based on constitutive bacterial promoters as well as arsenite biosensors based on the arsenite-inducible P-ars promoter. We observed important variations of the bioluminescence emission levels in five different Escherichia coli strains harboring two different lux-based biosensors, suggesting that the best host strain has to be empirically selected for each new biosensor under construction. We also investigated the bioluminescence reporter gene system transferred into Deinococcus deserti, an environmental, desiccation- and radiation-tolerant bacterium that would reduce the manufacturing costs of bacterial biosensors for commercial water analyzers and open the field of biodetection in radioactive environments. We thus successfully obtained a cell survival biosensor and a metal biosensor able to detect a concentration as low as 100 nM of arsenite in D. deserti. We demonstrated that the arsenite biosensor resisted desiccation and remained functional after 7 days stored in air-dried D. deserti cells. We also report here the use of a new near-infrared (NIR) fluorescent reporter candidate, a bacteriophytochrome from the magnetotactic bacterium Magnetospirillum magneticum AMB-1, which showed a NIR fluorescent signal that remained optimal despite increasing sample turbidity, while in similar conditions, a drastic loss of the lux-based biosensors signal was observed.
- Subjects :
- 0301 basic medicine
Arsenites
Health, Toxicology and Mutagenesis
Biosensing Techniques
macromolecular substances
[SDV.BC]Life Sciences [q-bio]/Cellular Biology
010501 environmental sciences
medicine.disease_cause
01 natural sciences
Gene Expression Regulation, Enzymologic
Microbiology
[ SDE ] Environmental Sciences
03 medical and health sciences
chemistry.chemical_compound
Genes, Reporter
Metals, Heavy
Escherichia coli
medicine
Environmental Chemistry
Bioluminescence
Deinococcus
[SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology
Promoter Regions, Genetic
[ SDV.BBM ] Life Sciences [q-bio]/Biochemistry, Molecular Biology
0105 earth and related environmental sciences
Arsenite
Reporter gene
[SDV.GEN]Life Sciences [q-bio]/Genetics
biology
[ SDV.BC ] Life Sciences [q-bio]/Cellular Biology
technology, industry, and agriculture
Water
Gene Expression Regulation, Bacterial
General Medicine
biology.organism_classification
Deinococcus deserti
Pollution
Luciferases, Bacterial
030104 developmental biology
chemistry
Biochemistry
[SDE]Environmental Sciences
[ SDV.GEN ] Life Sciences [q-bio]/Genetics
Biosensor
Water Pollutants, Chemical
Bacteria
Environmental Monitoring
Subjects
Details
- Language :
- English
- ISSN :
- 09441344 and 16147499
- Database :
- OpenAIRE
- Journal :
- Environmental Science and Pollution Research, Environmental Science and Pollution Research, Springer Verlag, 2017, 24 (1), pp.52--65. ⟨10.1007/s11356-016-6952-2⟩, Environmental Science and Pollution Research, Springer Verlag, 2017, 24 (1), pp.52--65. 〈10.1007/s11356-016-6952-2〉, Environmental Science and Pollution Research, 2017, 24 (1), pp.52--65. ⟨10.1007/s11356-016-6952-2⟩
- Accession number :
- edsair.doi.dedup.....fe791429a1e5c9f68028046a07f18e4c