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WOX13-like genes are required for reprogramming of leaf and protoplast cells into stem cells in the moss Physcomitrella patens
- Source :
- Development. 141:1660-1670
- Publication Year :
- 2014
- Publisher :
- The Company of Biologists, 2014.
-
Abstract
- Many differentiated plant cells can dedifferentiate into stem cells, reflecting the remarkable developmental plasticity of plants. In the moss Physcomitrella patens, cells at the wound margin of detached leaves become reprogrammed into stem cells. Here, we report that two paralogous P. patens WUSCHEL-related homeobox 13-like (PpWOX13L) genes, homologs of stem cell regulators in flowering plants, are transiently upregulated and required for the initiation of cell growth during stem cell formation. Concordantly, Δppwox13l deletion mutants fail to upregulate genes encoding homologs of cell wall loosening factors during this process. During the moss life cycle, most of the Δppwox13l mutant zygotes fail to expand and initiate an apical stem cell to form the embryo. Our data show that PpWOX13L genes are required for the initiation of cell growth specifically during stem cell formation, in analogy to WOX stem cell functions in seed plants, but using a different cellular mechanism.
- Subjects :
- Plant stem cell
Zygote
Cellular differentiation
Physcomitrella
Meristem
Molecular Sequence Data
Arabidopsis
Biology
Genes, Plant
Physcomitrella patens
Cell Wall
Gene Expression Regulation, Plant
Regeneration
Amino Acid Sequence
Molecular Biology
Cell Proliferation
Plant Proteins
Genetics
Arabidopsis Proteins
Cell growth
Protoplasts
Stem Cells
food and beverages
Plant cell
biology.organism_classification
Bryopsida
Up-Regulation
Cell biology
Plant Leaves
Stem cell
Reprogramming
Gene Deletion
Developmental Biology
Subjects
Details
- ISSN :
- 14779129 and 09501991
- Volume :
- 141
- Database :
- OpenAIRE
- Journal :
- Development
- Accession number :
- edsair.doi.dedup.....fe752d68fb0b9275d9df344f06cfeebb