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Suppressed ABA signal transduction in the spike promotes sucrose use in the stem and reduces grain number in wheat under water stress
- Source :
- Journal of Experimental Botany
- Publication Year :
- 2020
- Publisher :
- Oxford University Press (OUP), 2020.
-
Abstract
- Water stress is a primary trigger for reducing grain number per spike in wheat during the reproductive period. However, under stress conditions, the responses of plant organs and the interactions between them at the molecular and physiological levels remain unclear. In this study, when water stress occurred at the young microspore stage, RNA-seq data indicated that the spike had 970 differentially expressed genes, while the stem, comprising the two internodes below the spike (TIS), had 382. Abscisic acid (ABA) signal transduction genes were down-regulated by water stress in both these tissues, although to a greater extent in the TIS than in the spike. A reduction in sucrose was observed, and was accompanied by increases in cell wall invertase (CWIN) and sucrose:sucrose 1-fructosyl-transferase (1-SST) activities. Hexose and fructan were increased in the TIS but decreased in the spike. ABA was increased in the spike and TIS, and showed significant positive correlation with CWIN and 1-SST activities in the TIS. Overall, our results suggest that water stress induces the conversion of sucrose to hexose by CWIN, and to fructan by 1-SST, due to increased down-regulation of ABA signal transduction related-genes in the TIS; this leads to deficient sucrose supply to the spike and a decrease in grain number.<br />Deficient sucrose and weak expression of ABA signal transduction genes in the spike may be the major reasons for the decrease in grain number under water stress.
- Subjects :
- grain number per spike
0106 biological sciences
0301 basic medicine
Sucrose
Physiology
Plant Science
01 natural sciences
water stress
03 medical and health sciences
chemistry.chemical_compound
Fructan
Microspore
Gene Expression Regulation, Plant
wheat
sucrose metabolism enzyme
Endogenous hormones
Hexose
Abscisic acid
Triticum
Plant stem
chemistry.chemical_classification
Dehydration
AcademicSubjects/SCI01210
food and beverages
Research Papers
Cell biology
030104 developmental biology
Invertase
water-soluble carbohydrates
chemistry
Plant—Environment Interactions
Signal transduction
transcriptome
signal transduction
Abscisic Acid
010606 plant biology & botany
Subjects
Details
- ISSN :
- 14602431 and 00220957
- Volume :
- 71
- Database :
- OpenAIRE
- Journal :
- Journal of Experimental Botany
- Accession number :
- edsair.doi.dedup.....fe606728a852de53d4d34c0c844eb863