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Evaluation of TLR9 expression on PBMCs and CpG ODN-TLR9 ligation on IFN-α production in SLE patients
- Source :
- Immunopharmacology and immunotoxicology. 39(1)
- Publication Year :
- 2017
-
Abstract
- Systemic lupus erythematosus (SLE) is a systemic autoimmune disease characterized by autoreactive antibodies. Recent findings revealed the importance of innate immune responses, especially Toll-like receptors (TLRs) in the pathogenesis of SLE.In this study, the level of TLR9 expression on peripheral blood mononuclear cells (PBMCs) was analyzed. The levels of produced IFN-α were also measured in supernatant of PBMCs from SLE patients and healthy controls after stimulation with CpG ODN2216 which is a plasmocytoid dendritic cell (pDC)-specific TLR9 ligand.TLR9 expression was analyzed by real-time polymerase chain reaction (PCR) and flow cytometry in 35 SLE patients and 38 healthy controls and IFN-α concentration was measured in supernatants using enzyme-linked immunosorbent assay (ELISA).The results showed that the TLR9 expression in the mRNA and the protein level was significantly higher in PBMCs from SLE patients. However, IFN-α concentration in patients and controls significantly increased in response to CpG stimulation but this increase was significantly higher in healthy controls compared with SLE patients. Our results do not show any association between taking hydroxychloroquine and reduction in IFN-α production in SLE patients.Regarding the findings of the study, there is the possibility that TLR9 has played a role in SLE pathogenesis, and consequently it implies that TLRs can be considered to be the therapeutic targets for systemic autoimmunity. We may conclude that PBMCs in patients are functionally impaired in response to TLR ligation via innate response stimulating pathogen-associated molecular patterns (PAMPs).
- Subjects :
- 0301 basic medicine
Adult
Male
Anti-nuclear antibody
CpG Oligodeoxynucleotide
Immunology
Toxicology
Peripheral blood mononuclear cell
Flow cytometry
Pathogenesis
03 medical and health sciences
0302 clinical medicine
immune system diseases
medicine
Immunology and Allergy
Humans
Lupus Erythematosus, Systemic
skin and connective tissue diseases
Aged
030203 arthritis & rheumatology
Pharmacology
biology
medicine.diagnostic_test
TLR9
Interferon-alpha
hemic and immune systems
General Medicine
Dendritic cell
Middle Aged
Molecular biology
030104 developmental biology
Gene Expression Regulation
Oligodeoxyribonucleotides
Toll-Like Receptor 9
biology.protein
Leukocytes, Mononuclear
Female
Antibody
Subjects
Details
- ISSN :
- 15322513
- Volume :
- 39
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Immunopharmacology and immunotoxicology
- Accession number :
- edsair.doi.dedup.....fe48ffef11420e889920e54d7c1205ca