Back to Search
Start Over
Hypoxia-induced expression of VE-cadherin and Filamin B in gliomacell cultures and pseudopalisade structures
- Source :
- Journal of Neuro-Oncology, Journal of Neuro-Oncology, Springer Verlag, 2013, pp.1124-4. ⟨10.1007/s11060-013-1124-4⟩, Journal of Neuro-Oncology, Springer Verlag, 2013, 113 (2), pp.239-49. ⟨10.1007/s11060-013-1124-4⟩
- Publication Year :
- 2013
- Publisher :
- Springer, 2013.
-
Abstract
- International audience; Most of our knowledge regarding glioma cell biology comes from cell culture experiments. For many years the standards for glioma cell culture were the use of cell lines cultured in the presence of serum and 20 % O2. However, in vivo, normoxia in many brain areas is in close to 3 % O2. Hence, in cell culture, the experimental value referred as the norm is hyperoxic compared to any brain physiological value. Likewise, cells in vivo are not usually exposed to serum, and low-passaged glioma neurosphere cultures maintained in serum-free medium is emerging as a new standard. A consequence of changing the experimental normoxic standard from 20 % O2 to the more brain physiological value of 3 % O2, is that a 3 % O2 normoxic reference point enabled a more rigorous characterization of the level of regulation of genes by hypoxia. Among the glioma hypoxia-regulated genes characterized using this approach we found VE-cadherin that is required for blood vessel formation, and filamin B a gene involved in endothelial cell motility. Both VE-cadherin and filamin B were found expressed in pseudopalisades, a glioblastoma pathognomonic structure made of hypoxic migrating cancer cells. These results provide additional clues on the role played by hypoxia in the acquisition of endothelial traits by glioma cells and on the functional links existing between pseudopalisades, hypoxia, and tumor progression.
- Subjects :
- Cancer Research
Pathology
Filamin
Immunoenzyme Techniques
0302 clinical medicine
VE-cadherin
Cell Movement
Tumor Cells, Cultured
Hypoxia
Oligonucleotide Array Sequence Analysis
0303 health sciences
Brain Neoplasms
Reverse Transcriptase Polymerase Chain Reaction
Glioma
Cadherins
Filamin B
Endothelial stem cell
Neurology
Oncology
pseudopalisade
030220 oncology & carcinogenesis
medicine.medical_specialty
Filamins
Blotting, Western
[SDV.CAN]Life Sciences [q-bio]/Cancer
Biology
Real-Time Polymerase Chain Reaction
Article
03 medical and health sciences
Necrosis
Antigens, CD
Neurosphere
medicine
Biomarkers, Tumor
Humans
RNA, Messenger
030304 developmental biology
Cell Proliferation
Cadherin
Cell growth
Gene Expression Profiling
medicine.disease
Cell culture
Cancer cell
Cancer research
Neurology (clinical)
Endothelium, Vascular
endothelial to mesenchymal transition
[SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Subjects
Details
- Language :
- English
- ISSN :
- 0167594X and 15737373
- Database :
- OpenAIRE
- Journal :
- Journal of Neuro-Oncology, Journal of Neuro-Oncology, Springer Verlag, 2013, pp.1124-4. ⟨10.1007/s11060-013-1124-4⟩, Journal of Neuro-Oncology, Springer Verlag, 2013, 113 (2), pp.239-49. ⟨10.1007/s11060-013-1124-4⟩
- Accession number :
- edsair.doi.dedup.....fe438fcb501634e4e1af1df7beabc574