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Adjuvant activity of Mycobacterium bovis BCG expressing CRM197 on the immune response induced by BCG expressing tetanus toxin fragment C
- Source :
- Vaccine, Vaccine, Elsevier, 2004, 22 (5-6), pp.740-746. ⟨10.1016/j.vaccine.2003.08.017⟩, Vaccine, 2004, 22 (5-6), pp.740-746. ⟨10.1016/j.vaccine.2003.08.017⟩
- Publication Year :
- 2004
- Publisher :
- HAL CCSD, 2004.
-
Abstract
- International audience; In order to develop a combined recombinant Mycobacterium bovis BCG (rBCG) vaccine against diphtheria, pertussis and tetanus (DPT), we have constructed different strains of rBCG expressing tetanus toxin fragment C (FC), driven by the up-regulated M. fortuitum beta-lactamase promoter, pBlaF*. Tetanus toxin FC was expressed in comparable levels in native form or in fusion with the beta-lactamase exportation signal sequence; however, in both constructs it was localized to the cytosol. Immunization of mice with rBCG-FC or its combination with rBCG expressing CRM197, induced anti-tetanus toxin antibodies with a Th2 immunoglobulin profile. Administration of a subimmunizing dose of the diphtheria-tetanus toxoid vaccine showed that rBCG-FC primed mice for production of an intense humoral response. Interestingly, the combination of rBCG-FC and rBCG-CRM197 reduced the time required for maturation of the immune response and increased anti-tetanus toxin antibody levels, suggesting adjuvant properties for rBCG-CRM197; this combination induced 75% protection in mice challenged with 100 minimum lethal doses (MLD) of tetanus toxin. Antisera from guinea pigs immunized with this combination were shown to neutralize tetanus toxin and diphtheria toxin. Our results suggest reciprocal adjuvant effects of rBCG-FC and rBCG-CRM197, which may contribute to induction of a more effective immune response against both diseases.
- Subjects :
- Male
medicine.medical_treatment
medicine.disease_cause
immune response
Chlorocebus aethiops
INFECTION
Diphtheria Toxin
0303 health sciences
Mice, Inbred BALB C
Vaccines, Synthetic
biology
Tetanus
PROTECTIVE IMMUNITY
Antibodies, Bacterial
Mycobacterium bovis
PERTUSSIS
3. Good health
Infectious Diseases
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology
ESCHERICHIA-COLI
PHOA GENE FUSIONS
BCG Vaccine
Molecular Medicine
Antibody
Adjuvant
Blotting, Western
Genetic Vectors
DIPHTHERIA-TOXIN
Enzyme-Linked Immunosorbent Assay
complex mixtures
Microbiology
03 medical and health sciences
Immune system
Adjuvants, Immunologic
Bacterial Proteins
Tetanus Toxin
SURFACE PROTEIN-A
Neutralization Tests
medicine
Escherichia coli
Animals
Vaccines, Combined
Serotyping
Vero Cells
Diphtheria-Tetanus-Pertussis Vaccine
030304 developmental biology
tetanus toxin fragment C
Diphtheria toxin
General Veterinary
General Immunology and Microbiology
030306 microbiology
Toxin
Diphtheria
Public Health, Environmental and Occupational Health
medicine.disease
Virology
MICE
Immunization
Antibody Formation
ANTIBODIES
biology.protein
RECOMBINANT VACCINES
Subjects
Details
- Language :
- English
- ISSN :
- 0264410X
- Database :
- OpenAIRE
- Journal :
- Vaccine, Vaccine, Elsevier, 2004, 22 (5-6), pp.740-746. ⟨10.1016/j.vaccine.2003.08.017⟩, Vaccine, 2004, 22 (5-6), pp.740-746. ⟨10.1016/j.vaccine.2003.08.017⟩
- Accession number :
- edsair.doi.dedup.....fe3e8bfeadd7b80b81fc37ec283e4a01