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Purification, characterization and immunomodulating activity of a polysaccharide from flowers of Abelmoschus esculentus
- Source :
- Carbohydrate polymers. 106
- Publication Year :
- 2014
-
Abstract
- A water-soluble polysaccharide (OFPS11) was obtained from okra (Abelmoschus esculentus) flowers using aqueous extraction and purification with DEAE-52 cellulose and Sephacryl™ S-500 column. Its preliminary characterization and immunomodulating activity were investigated. Results showed that OFPS11 is mainly composed of galactose and rhamnose in a molar ratio of 2.23:1 with molecular mass of 1,700 kDa. RAW264.7 cells pretreated with OFPS11 significantly inhibited the proliferation of HepG-2 cells. Additionally, OFPS11 enhanced the phagocytic ability and induced the elevation of NO production, TNF-α and IL-1β secretion of RAW264.7 cells. Furthermore, OFPS11 promoted both the expression of iNOS protein and of iNOS and TNF-α mRNA. OFPS11 can strongly increase NF-κB levels in nucleuses, which is an important transcription factor that can modulate expressions of iNOS, NO and TNF-α. These outcomes support that OFPS11 exerts its antitumor activity by probably stimulating macrophage activities through nuclear NF-κB pathway.
- Subjects :
- Cytotoxicity, Immunologic
Polymers and Plastics
Transcription, Genetic
Rhamnose
Drug Evaluation, Preclinical
Nitric Oxide Synthase Type II
Flowers
Polysaccharide
Nitric Oxide
Cell Line
chemistry.chemical_compound
Mice
Phagocytosis
Abelmoschus
Polysaccharides
Materials Chemistry
Animals
Humans
Immunologic Factors
Secretion
RNA, Messenger
Cellulose
Transcription factor
chemistry.chemical_classification
Cell Nucleus
Molecular mass
biology
Tumor Necrosis Factor-alpha
Macrophages
Organic Chemistry
NF-kappa B
Water
Hep G2 Cells
biology.organism_classification
Antineoplastic Agents, Phytogenic
Up-Regulation
Molecular Weight
chemistry
Biochemistry
Solubility
Galactose
Subjects
Details
- ISSN :
- 18791344
- Volume :
- 106
- Database :
- OpenAIRE
- Journal :
- Carbohydrate polymers
- Accession number :
- edsair.doi.dedup.....fe213a072f7215eda44b1a9a39e0879b