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Adenovirus-Mediated Wnt10b Overexpression Induces Hair Follicle Regeneration
- Source :
- Journal of Investigative Dermatology. 133:42-48
- Publication Year :
- 2013
- Publisher :
- Elsevier BV, 2013.
-
Abstract
- Hair follicles periodically undergo regeneration. The balance between activators and inhibitors may determine the time required for telogen hair follicles to reenter anagen. We previously reported that Wnt10b (wingless-type mouse mammary tumor virus integration site family member 10b) could promote the growth of hair follicles in vitro. To unveil the roles of Wnt10b in hair follicle regeneration, we established an in vivo mouse model using intradermal injection. On the basis of this model, we found that Wnt10b could induce the biological switch of hair follicles from telogen to anagen when overexpressed in the skin. The induced hair follicles expressed structure markers and could cycle normally into catagen. Conversely, anagen onset was abrogated by the knockdown of Wnt10b with small interfering RNA (siRNA). The Wnt10b aberrant expression data suggest that it is one of the activators of hair follicle regeneration. The β-catenin protein is translocated to the nucleus in Wnt10b-induced hair follicles. The biological effects of Wnt10b were abrogated when β-catenin expression was downregulated with siRNA. These data revealed that Wnt10b might induce hair follicle regeneration in vivo via the enhanced activation of the canonical Wnt signaling pathway. To our knowledge, our data provide previously unreported insights into the regulation of hair follicle cycling and provide potential therapeutic targets for hair follicle–related diseases.
- Subjects :
- Gene Expression Regulation, Viral
medicine.medical_specialty
Small interfering RNA
Genetic Vectors
Active Transport, Cell Nucleus
Dermatology
Biochemistry
Adenoviridae
Mice
In vivo
Internal medicine
medicine
Animals
Regeneration
Gene Silencing
Intradermal injection
Wnt Signaling Pathway
Molecular Biology
beta Catenin
Gene knockdown
integumentary system
biology
Regeneration (biology)
Mouse mammary tumor virus
Cell Biology
Hair follicle
biology.organism_classification
In vitro
Up-Regulation
Cell biology
Mice, Inbred C57BL
Wnt Proteins
medicine.anatomical_structure
Endocrinology
Female
sense organs
Hair Follicle
Subjects
Details
- ISSN :
- 0022202X
- Volume :
- 133
- Database :
- OpenAIRE
- Journal :
- Journal of Investigative Dermatology
- Accession number :
- edsair.doi.dedup.....fe1e91793a1353dc22967909c4cf0e8f