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Nucleotide sequences of the variable regions of a human monoclonal antibody against HLA-A1, A23, and A24
- Source :
- Tissue Antigens. 41:249-254
- Publication Year :
- 1993
- Publisher :
- Wiley, 1993.
-
Abstract
- Nucleotide sequences of the heavy and light chain variable (VH and VL) regions of a human monoclonal antibody (4-35-7), which recognized HLA-A1, A23 and A24, were determined by means of the reverse transcriptase-polymerase chain reaction. This antibody was generated by Epstein-Barr virus transformation of lymphocytes obtained from a multiparous donor, followed by fusion with mouse myeloma cells. The VH gene segment belonged to the VHIII gene family, and used the DXP4 and JH4 gene segments. This VH gene segment had 92.9% homology to the germline gene VH26, and contained 21 nucleotide substitutions. Fourteen of them generated the replacements of amino acids, while 7 failed to generate the replacement. The ratio of replacement to silent mutations in complementarity determining regions (CDRs) was 7.0. The VL gene segment belonged to the VkI gene family, and used Jk4. This VL gene segment showed 96.1% homology to the germline gene HK102, and contained 11 nucleotide substitutions. Seven of them generated the replacement of amino acids, while 4 failed to generate the replacement. The high ratio of replacement to silent mutations in CDRs of the VH gene segment suggested that the multiparity caused the processes of antigenic selection and somatic mutation, and generated this anti-HLA antibody.
- Subjects :
- Silent mutation
Molecular Sequence Data
Immunology
Immunoglobulin Variable Region
HLA-A24 Antigen
Complementarity determining region
Biology
Immunoglobulin light chain
Polymerase Chain Reaction
Biochemistry
Homology (biology)
Germline mutation
Antibody Specificity
Genetics
Humans
Immunology and Allergy
Gene family
Amino Acid Sequence
Gene
HLA-A1 Antigen
Base Sequence
Genes, Immunoglobulin
HLA-A Antigens
Sequence Homology, Amino Acid
Nucleic acid sequence
Antibodies, Monoclonal
RNA-Directed DNA Polymerase
General Medicine
Molecular biology
Immunoglobulin Light Chains
Immunoglobulin Heavy Chains
Sequence Alignment
Subjects
Details
- ISSN :
- 13990039 and 00012815
- Volume :
- 41
- Database :
- OpenAIRE
- Journal :
- Tissue Antigens
- Accession number :
- edsair.doi.dedup.....fe1b90840d31323fefcf496e3aa3fc89