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Direct Molecular Detection and Genotyping of Borrelia burgdorferi from Whole Blood of Patients with Early Lyme Disease
- Source :
- PLoS ONE, PLoS ONE, Vol 7, Iss 5, p e36825 (2012)
- Publication Year :
- 2012
- Publisher :
- Public Library of Science (PLoS), 2012.
-
Abstract
- Direct molecular tests in blood for early Lyme disease can be insensitive due to low amount of circulating Borrelia burgdorferi DNA. To address this challenge, we have developed a sensitive strategy to both detect and genotype B. burgdorferi directly from whole blood collected during the initial patient visit. This strategy improved sensitivity by employing 1.25 mL of whole blood, a novel pre-enrichment of the entire specimen extract for Borrelia DNA prior to a multi-locus PCR and electrospray ionization mass spectrometry detection assay. We evaluated the assay on blood collected at the initial presentation from 21 endemic area patients who had both physician-diagnosed erythema migrans (EM) and positive two-tiered serology either at the initial visit or at a follow-up visit after three weeks of antibiotic therapy. Results of this DNA analysis showed detection of B. burgdorferi in 13 of 21 patients (62%). In most cases the new assay also provided the B. burgdorferi genotype. The combined results of our direct detection assay with initial physician visit serology resulted in the detection of early Lyme disease in 19 of 21 (90%) of patients at the initial visit. In 5 of 21 cases we demonstrate the ability to detect B. burgdorferi in early Lyme disease directly from whole blood specimens prior to seroconversion.
- Subjects :
- Bacterial Diseases
DNA, Bacterial
Male
Genotype
Epidemiology
Science
Biology
Polymerase Chain Reaction
Infectious Disease Epidemiology
Serology
Molecular Genetics
03 medical and health sciences
Lyme disease
Diagnostic Medicine
Borrelia
Genetics
medicine
Humans
Seroconversion
Borrelia burgdorferi
Genotyping
030304 developmental biology
Whole blood
Lyme Disease
0303 health sciences
Multidisciplinary
Population Biology
030306 microbiology
bacterial infections and mycoses
medicine.disease
biology.organism_classification
Borrelia Infection
Antibodies, Bacterial
Virology
Glossitis, Benign Migratory
3. Good health
Infectious Diseases
Immunology
Medicine
Lyme disease microbiology
Female
Research Article
Follow-Up Studies
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 7
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....fe18ab6a2fb08ee8fd7c653228affd31