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Development of a Real-Time Quantitative RT-PCR Assay for Detection of Bovine Rhinitis B Virus
- Source :
- Frontiers in Veterinary Science, Vol 8 (2021), Frontiers in Veterinary Science
- Publication Year :
- 2021
- Publisher :
- Frontiers Media S.A., 2021.
-
Abstract
- Bovine rhinitis B virus (BRBV) has been frequently identified in cattle diagnosed with bovine respiratory disease complex (BRDC) in recent years, suggesting its potential contribution to BRDC. The goal of this study was to develop a TaqMan-based real-time quantitative RT-PCR assay for efficient BRBV detection. A pair of primers and a probe were designed based on the 3D gene of the BRBV genome. The assay was specific for BRBV and able to exclude bovine rhinitis A virus, foot-and-mouth disease virus and Senecavirus A. The limit of detection of the assay was 4.46 copies per reaction. A standard curve was plotted, with a coefficient of determination of 0.999 in the concentration range of 100-108 copies/μl. The reproducibility of the assay was acceptable, with the standard deviations of cycle threshold values lower than 1.00 in both intra- and inter-assay. Of 200 samples collected from 150 head of cattle in recent years in China, 11% (22/200) of the samples tested positive in the assay, i.e., 4.6% (7/150) of the cattle were BRBV positive. This study provides an efficient diagnostic tool for the epidemiological investigations of BRBV.
- Subjects :
- Detection limit
0303 health sciences
Cycle threshold
General Veterinary
040301 veterinary sciences
Veterinary medicine
RT-qPCR
detection
04 agricultural and veterinary sciences
Biology
assay
bovine rhinitis B virus
Virology
Virus
0403 veterinary science
Standard curve
TaqMan probe
03 medical and health sciences
Real-time polymerase chain reaction
SF600-1100
Methods
TaqMan
Veterinary Science
Bovine rhinitis B virus
030304 developmental biology
Subjects
Details
- Language :
- English
- ISSN :
- 22971769
- Volume :
- 8
- Database :
- OpenAIRE
- Journal :
- Frontiers in Veterinary Science
- Accession number :
- edsair.doi.dedup.....fdfb8d3cb14b80ddc1d659b92b3914b2