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miR-29b-3p inhibits post-infarct cardiac fibrosis by targeting FOS
- Source :
- Bioscience Reports
- Publication Year :
- 2020
- Publisher :
- Portland Press Ltd., 2020.
-
Abstract
- Background: Cardiac fibrosis after myocardial infarction (MI) is a major cause of heart deterioration. Recently, the roles of microRNAs (miRNAs) in various cardiovascular diseases associated with cardiac fibrosis have been extensively investigated. The present study aimed to investigate the role and mechanism of miR-29b-3p in cardiac fibrosis after MI. Methods: miR-29b-3p expression in TGF-β1-activated cardiac fibroblasts (CFs) was detected by qRT-PCR. Cell Counting Kit-8 (CCK-8) and Trans-well assays were performed to evaluate CFs proliferation and migration ability, respectively. Protein expressions of α-SMA, collagen I, collagen III, MMP2, and MMP9 were examined by Western blot assay. Bioinformatics, luciferase, and RNA immunoprecipitation (RIP) assays were carried out to determine whether FOS was targeted by miR-29b-3p. Results: TGF-β1 treatment dose-dependently curbed miR-29b-3p expression in CFs. miR-29b-3p restrained the promotive impacts of TGF-β1 on CFs proliferation, migration, and differentiation. FOS was affirmed to be a target of miR-29b-3p, elevated expression of FOS reversed the inhibitory effects of miR-29b-3p on cell proliferation, migration, and differentiation in TGF-β1-activated CFs. Conclusion: miR-29b-3p degraded the pro-fibrosis effect induced by TGF-β1 via targeting FOS, providing a prospective therapeutic avenue for cardiac fibrosis after MI.
- Subjects :
- 0301 basic medicine
MMP2
Cardiac fibrosis
proliferation
cardiac fibrosis
Biophysics
Myocardial Infarction
MMP9
Inhibitory postsynaptic potential
migration
Biochemistry
Rats, Sprague-Dawley
Transforming Growth Factor beta1
03 medical and health sciences
0302 clinical medicine
Cell Movement
microRNA
Medicine
Animals
Luciferase
Myocardial infarction
Molecular Biology
Research Articles
Cell Proliferation
miR-29b-3p
Cell growth
business.industry
FOS
Myocardium
Cell Differentiation
Heart
Cell Biology
differentiation
Fibroblasts
medicine.disease
Fibrosis
MicroRNAs
030104 developmental biology
030220 oncology & carcinogenesis
Gene Knockdown Techniques
Cancer research
business
Proto-Oncogene Proteins c-fos
Biotechnology
Subjects
Details
- Language :
- English
- ISSN :
- 15734935 and 01448463
- Volume :
- 40
- Issue :
- 9
- Database :
- OpenAIRE
- Journal :
- Bioscience Reports
- Accession number :
- edsair.doi.dedup.....fdf9016618b5b5a12ce3c4b2c716a9f8