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A five‐miRNA panel in plasma was identified for breast cancer diagnosis
- Source :
- Cancer Medicine, Cancer Medicine, Vol 8, Iss 16, Pp 7006-7017 (2019)
- Publication Year :
- 2019
- Publisher :
- Wiley, 2019.
-
Abstract
- Breast cancer (BC) is one of the most common cancers in females. Since early detection can bring prognosis benefit, discovery of novel noninvasive biomarkers for BC diagnosis is in urgent need. In this four‐phase study, we profiled miRNA expression in plasma samples from a total of 257 BC patients and 257 normal controls (NCs). Exiqon miRNA qPCR panel was used to select candidate miRNAs in the screening phase which were further analyzed using qRT‐PCR in the following training, testing and external validation phases. Finally, we identified five plasma miRNAs (let‐7b‐5p, miR‐122‐5p, miR‐146b‐5p, miR‐210‐3p and miR‐215‐5p) whose expression levels were significantly different between BC patients and NCs. A 5‐miRNA panel in plasma with high sensitivity and specificity was then constructed to detect BC. The areas under the receiver‐operating characteristic curves (AUCs) of the panel were 0.683, 0.966, 0.978 for the training, testing and external validation sets, respectively. Expression of the identified miRNAs was further analyzed among 32 pairs of BC tissue and the adjacent normal tissue samples as well as plasma‐derived exosome samples from 32 BC patients vs 32 NCs. Let‐7b‐5p was contrarily down‐regulated in BC tissue. In exosomes samples, only miR‐122‐5p was significantly up‐regulated as in plasma for BC patients. In conclusion, we identified a 5‐miRNA plasma panel (let‐7b‐5p, miR‐122‐5p, miR‐146b‐5p, miR‐210‐3p and miR‐215‐5p) that could serve as a promising biomarker for BC detection.<br />We identified five plasma miRNAs (let‐7b‐5p, miR‐122‐5p, miR‐146b‐5p, miR‐210‐3p and miR‐215‐5p) by multiple‐phase validation which could serve as novel noninvasive biomarkers for BC diagnosis. Our findings may contribute to the early detection of BC and be beneficial to BC patients.
- Subjects :
- 0301 basic medicine
Oncology
Cancer Research
medicine.medical_specialty
diagnosis
Normal tissue
Breast Neoplasms
Exosomes
lcsh:RC254-282
Exosome
03 medical and health sciences
breast cancer
0302 clinical medicine
Breast cancer
plasma miRNA
Internal medicine
microRNA
Biomarkers, Tumor
medicine
Humans
Radiology, Nuclear Medicine and imaging
Circulating MicroRNA
Original Research
Noninvasive biomarkers
business.industry
Clinical Cancer Research
qRT‐PCR
Middle Aged
lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens
medicine.disease
Microvesicles
030104 developmental biology
Real-time polymerase chain reaction
030220 oncology & carcinogenesis
biomarker
Biomarker (medicine)
Female
business
Subjects
Details
- ISSN :
- 20457634
- Volume :
- 8
- Database :
- OpenAIRE
- Journal :
- Cancer Medicine
- Accession number :
- edsair.doi.dedup.....fdf8c9f1bfcfd50d7b303d64f4457b54