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A new intra-specific and high-resolution genetic map of eggplant based on a ril population, and location of QTLS related to plant anthocyanin pigmentation and seed vigour
- Source :
- Genes, Volume 11, Issue 7, Genes, Vol 11, Iss 745, p 745 (2020), Genes (Basel) 11 (2020): 1–29. doi:10.3390/genes11070745, info:cnr-pdr/source/autori:Toppino L, Barchi L, Mercati F, Acciarri N, Perrone D, Martina M, Gattolin S, Sala T, Fadda S, Mauceri A, Ciriaci T, Carimi F, Portis E, Sunseri F, Lanteri S, Rotino GL/titolo:A New Intra-Specific and High-Resolution Genetic Map of Eggplant based on a RIL Population, and Location of QTLs Related to Plant Anthocyanin Pigmentation and Seed Vigour/doi:10.3390%2Fgenes11070745/rivista:Genes (Basel)/anno:2020/pagina_da:1/pagina_a:29/intervallo_pagine:1–29/volume:11
- Publication Year :
- 2020
-
Abstract
- Eggplant is the second most important solanaceous berry-producing crop after tomato. Despite mapping studies based on bi-parental progenies and GWAS approaches having been performed, an eggplant intraspecific high-resolution map is still lacking. We developed a RIL population from the intraspecific cross &lsquo<br />305E40&prime<br />(androgenetic introgressed line carrying the locus Rfo-Sa1 conferring Fusarium resistance) x &lsquo<br />67/3&prime<br />(breeding line whose genome sequence was recently released). One hundred and sixty-three RILs were genotyped by a genotype-by-sequencing (GBS) approach, which allowed us to identify 10,361 polymorphic sites. Overall, 267 Gb of sequencing data were generated and ~773 M Illumina paired end (PE) reads were mapped against the reference sequence. A new linkage map was developed, including 7249 SNPs assigned to the 12 chromosomes and spanning 2169.23 cM, with iaci@liberoan average distance of 0.4 cM between adjacent markers. This was used to elucidate the genetic bases of seven traits related to anthocyanin content in different organs recorded in three locations as well as seed vigor. Overall, from 7 to 17 QTLs (at least one major QTL) were identified for each trait. These results demonstrate that our newly developed map supplies valuable information for QTL fine mapping, candidate gene identification, and the development of molecular markers for marker assisted selection (MAS) of favorable alleles.
- Subjects :
- 0106 biological sciences
0301 basic medicine
lcsh:QH426-470
Genetic Linkage
QTL
Population
Quantitative Trait Loci
Locus (genetics)
Quantitative trait locus
Biology
01 natural sciences
Article
Chromosomes, Plant
Anthocyanins
03 medical and health sciences
Fusarium
Genetic linkage
Genetics
Solanum melongena
Allele
education
Genetics (clinical)
Disease Resistance
2. Zero hunger
Whole genome sequencing
education.field_of_study
Pigmentation
Linkage map
food and beverages
RAD
Marker-assisted selection
lcsh:Genetics
030104 developmental biology
Seeds
010606 plant biology & botany
Reference genome
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Genes, Volume 11, Issue 7, Genes, Vol 11, Iss 745, p 745 (2020), Genes (Basel) 11 (2020): 1–29. doi:10.3390/genes11070745, info:cnr-pdr/source/autori:Toppino L, Barchi L, Mercati F, Acciarri N, Perrone D, Martina M, Gattolin S, Sala T, Fadda S, Mauceri A, Ciriaci T, Carimi F, Portis E, Sunseri F, Lanteri S, Rotino GL/titolo:A New Intra-Specific and High-Resolution Genetic Map of Eggplant based on a RIL Population, and Location of QTLs Related to Plant Anthocyanin Pigmentation and Seed Vigour/doi:10.3390%2Fgenes11070745/rivista:Genes (Basel)/anno:2020/pagina_da:1/pagina_a:29/intervallo_pagine:1–29/volume:11
- Accession number :
- edsair.doi.dedup.....fddef84bbf093b136013173e695afcfd