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Molecular Characterization of Caveolin Association with the Golgi Complex: Identification of a Cis-Golgi Targeting Domain in the Caveolin Molecule
- Source :
- ResearcherID, The Journal of Cell Biology
- Publication Year :
- 1999
- Publisher :
- Rockefeller University Press, 1999.
-
Abstract
- Caveolins are integral membrane proteins which are a major component of caveolae. In addition, caveolins have been proposed to cycle between intracellular compartments and the cell surface but the exact trafficking route and targeting information in the caveolin molecule have not been defined. We show that antibodies against the caveolin scaffolding domain or against the COOH terminus of caveolin-1 show a striking specificity for the Golgi pool of caveolin and do not recognize surface caveolin by immunofluorescence. To analyze the Golgi targeting of caveolin in more detail, caveolin mutants were expressed in fibroblasts. Specific mutants lacking the NH2 terminus were targeted to the cis Golgi but were not detectable in surface caveolae. Moreover, a 32–amino acid segment of the putative COOH-terminal cytoplasmic domain of caveolin-3 was targeted specifically and exclusively to the Golgi complex and could target a soluble heterologous protein, green fluorescent protein, to this compartment. Palmitoylation-deficient COOH-terminal mutants showed negligible association with the Golgi complex. This study defines unique Golgi targeting information in the caveolin molecule and identifies the cis Golgi complex as an intermediate compartment on the caveolin cycling pathway.
- Subjects :
- Caveolin 3
Recombinant Fusion Proteins
Caveolin 1
Molecular Sequence Data
Palmitic Acid
Fluorescent Antibody Technique
Golgi Apparatus
Protein Sorting Signals
Biology
Transfection
Caveolins
Antibodies
Cell Line
symbols.namesake
Caveolae
Caveolin
Animals
Humans
palmitoylation
Amino Acid Sequence
Microscopy, Immunoelectron
Integral membrane protein
Cells, Cultured
Conserved Sequence
targeting
Sequence Deletion
Nocodazole
Cell Membrane
Membrane Proteins
Golgi Targeting
Intracellular Membranes
Cell Biology
Fibroblasts
Golgi apparatus
Golgi complex
Cell biology
caveolae
symbols
caveolin
Fatty acylation
Protein Binding
Regular Articles
Subjects
Details
- ISSN :
- 15408140 and 00219525
- Volume :
- 145
- Database :
- OpenAIRE
- Journal :
- Journal of Cell Biology
- Accession number :
- edsair.doi.dedup.....fddb1f9a3ac176bd55ca2dda2d3668f2