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Kisspeptin-8 Induces Anxiety-Like Behavior and Hypolocomotion by Activating the HPA Axis and Increasing GABA Release in the Nucleus Accumbens in Rats
- Source :
- Biomedicines, Volume 9, Issue 2, Biomedicines, Vol 9, Iss 112, p 112 (2021)
- Publication Year :
- 2021
- Publisher :
- MDPI, 2021.
-
Abstract
- Kisspeptins (Kp) are RF-amide neuropeptide regulators of the reproductive axis that also influence anxiety, locomotion, and metabolism. We aimed to investigate the effects of intracerebroventricular Kp-8 (an N-terminally truncated octapeptide) treatment in Wistar rats. Elevated plus maze (EPM), computerized open field (OF), and marble burying (MB) tests were performed for the assessment of behavior. Serum LH and corticosterone levels were determined to assess kisspeptin1 receptor (Kiss1r) activation and hypothalamic-pituitary-adrenal axis (HPA) stimulation, respectively. GABA release from the nucleus accumbens (NAc) and dopamine release from the ventral tegmental area (VTA) and NAc were measured via ex vivo superfusion. Kp-8 decreased open arm time and entries in EPM, and also raised corticosterone concentration, pointing to an anxiogenic effect. Moreover, the decrease in arm entries in EPM, the delayed increase in immobility accompanied by reduced ambulatory activity in OF, and the reduction in interactions with marbles show that Kp-8 suppressed exploratory and spontaneous locomotion. The increase in GABA release from the NAc might be in the background of hypolocomotion by inhibiting the VTA-NAc dopaminergic circuitry. As Kp-8 raised LH concentration, it could activate Kiss1r and stimulate the reproductive axis. As Kiss1r is associated with hyperlocomotion, it is more likely that neuropeptide FF receptor activation is involved in the suppression of locomotor activity.
- Subjects :
- 0301 basic medicine
Elevated plus maze
medicine.medical_specialty
nucleus accumbens
Medicine (miscellaneous)
Neuropeptide FF receptor
Nucleus accumbens
Kiss1 receptor
General Biochemistry, Genetics and Molecular Biology
Open field
Article
Marble burying
kisspeptin
03 medical and health sciences
0302 clinical medicine
Internal medicine
medicine
lcsh:QH301-705.5
Chemistry
HPA axis
Dopaminergic
03.01. Általános orvostudomány
anxiety
Ventral tegmental area
locomotion
030104 developmental biology
medicine.anatomical_structure
Endocrinology
Anxiogenic
lcsh:Biology (General)
HPG axis
030217 neurology & neurosurgery
Subjects
Details
- Language :
- English
- ISSN :
- 22279059
- Volume :
- 9
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Biomedicines
- Accession number :
- edsair.doi.dedup.....fdd5f41ccb2e6d552232620648ceb24d