Chromatographic separation and evaluation of the lipophilicity by reversed-phase high-performance liquid chromatography of fullerene-C60 derivatives

Two water-soluble regioisomers of tris-dicarboxymethanofullerene-C60 with D3 (3) or C3 (4) symmetry have been shown to possess interesting neuroprotective properties, among which the free radical scavenging activity is particularly relevant. Here we report a faster preparative scale separation of the two trisadducts along with analytical RP-HPLC data of 3 and 4 in order to provide additional information for the evaluation of their membrane permeability.