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Coordinated activity of amoebic formin and profilin are essential for phagocytosis
- Source :
- Molecular Microbiology. 116:974-995
- Publication Year :
- 2021
- Publisher :
- Wiley, 2021.
-
Abstract
- For the protist parasite Entamoeba histolytica, endocytic processes, such as phagocytosis, are essential for its survival in the human gut. The actin cytoskeleton is involved in the formation of pseudopods and phagosomal vesicles by incorporating a number of actin-binding and modulating proteins along with actin in a temporal manner. The actin dynamics, which comprises polymerization, branching, and depolymerization is very tightly regulated and takes place directionally at the sites of initiation of phagocytosis. Formin and profilin are two actin-binding proteins that are known to regulate actin cytoskeleton dynamics and thereby, endocytic processes. In this article, we report the participation of formin and profilin in E. histolytica phagocytosis and propose that these two proteins interact with each other and their sequential recruitment at the site is required for the successful completion of phagocytosis. The evidence is based on detailed microscopic, live imaging, interaction studies, and expression downregulation. The cells downregulated for expression of formin show absence of profilin at the site of phagocytosis, whereas downregulation of profilin does not affect formin localization.
- Subjects :
- Phagocytosis
Endocytic cycle
Protozoan Proteins
Formins
CHO Cells
macromolecular substances
Microbiology
Profilins
Entamoeba histolytica
Cricetulus
Phagosomes
Animals
Humans
Molecular Biology
Actin
biology
Microfilament Proteins
biology.organism_classification
Actin cytoskeleton
Actins
Cell biology
Actin Cytoskeleton
Gene Expression Regulation
Profilin
biology.protein
Pseudopodia
Subjects
Details
- ISSN :
- 13652958 and 0950382X
- Volume :
- 116
- Database :
- OpenAIRE
- Journal :
- Molecular Microbiology
- Accession number :
- edsair.doi.dedup.....fd9b8cfbcc4a5191e9ff3b2734c76ae9