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Establishment of a Stable Cell Line Expressing Green Fluorescence Protein-fused Hypoxia Inducible Factor-1α for Assessment of Carcinogenicity of Chemical Toxicants
- Source :
- Toxicological Research
- Publication Year :
- 2009
- Publisher :
- Springer Singapore, 2009.
-
Abstract
- Hypoxia inducible factor 1α (HIF-1α) is a potential marker of carcicnogenesis since it is overexpresssed in many human cancers such as brain, breast, and uterus, and its role has implicated in tumor cell growth and metastasis. In this study, we established a stable cell line that express green fluorescence protein (GFP)-fused hypoxia inducible factor 1α (HIF-1α) and evaluated the potential use of this cell line for assessment of carcinogenicity of chemical toxicants. Western blot analysis as well as fluorescence measurements showed that protein-level of GFP-HIF-1α was significantly enhanced in a dose-dependent manner upon treatment of hypoxia mimicking agents such as dexferrioxamine and CoCl2. Well-Known tumor promoters such as mitomycin and methyl methane-sulfonate. significantly induced the fluorescence intensity of GFP-HIF-1α, whereas the known negative controls such as o-anthranilic acid and benzethonium chloride, did not. These results indicate that HIF-1α could be a biological parameter for detection of tumor initiators/promoters and suggest that the GFP-HIF-1α cell line is a useful system for screening of carcinogenic toxicants.
- Subjects :
- Chemical toxicants
Carcinogenicity
medicine.diagnostic_test
Health, Toxicology and Mutagenesis
Promoter
Hypoxia (medical)
Biology
Toxicology
medicine.disease
Molecular biology
GFP-HIF-1α stable cell line
Article
Green fluorescent protein
Metastasis
Hypoxia-inducible factors
Western blot
Cell culture
medicine
medicine.symptom
Carcinogen
Subjects
Details
- Language :
- English
- ISSN :
- 22342753 and 19768257
- Volume :
- 25
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Toxicological Research
- Accession number :
- edsair.doi.dedup.....fd734a5c10bc1e5396ac5ea53b60954b