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Inhibitory effects of 2-amino-3H-phenoxazin-3-one on the melanogenesis of murine B16 melanoma cell line
- Source :
- Bioscience, biotechnology, and biochemistry. 74(4)
- Publication Year :
- 2010
-
Abstract
- Hyperpigmentations are a serious concern addressed by both the medical community and the cosmetic industry through the development of agents that block melanin biosynthesis. In this study, we found that 2-amino-3H-phenoxazin-3-one (APO), isolated from extracts of the edible mushroom Agaricus bisporus Imbach, exhibited potent inhibitory effects on melanogenesis in B16 cells, a murine melanoma cell line. APO inhibited melanin biosynthesis at 1,000 times lower concentrations (IC(50)=1.31+/-0.08 microM) than kojic acid (IC(50)=1.31+/-0.13 mM), without causing cellular toxicity. APO did not directly inhibit the enzyme activity of tyrosinase, the rate-limiting melanogenic enzyme. Further study showed that APO inhibited the protein expression of tyrosinase and microphthalmia-associated transcription factor (MITF), a melanogenic transcription factor that regulates the expression of tyrosinase. These results suggest that APO is a promising depigmenting agent with both therapeutic and cosmetic value in preventing melanogenesis.
- Subjects :
- Indoles
Tyrosinase
Melanoma, Experimental
Applied Microbiology and Biotechnology
Biochemistry
Analytical Chemistry
Melanin
chemistry.chemical_compound
Mice
Cell Line, Tumor
Animals
Molecular Biology
Transcription factor
Melanoma
chemistry.chemical_classification
Melanins
Microphthalmia-Associated Transcription Factor
biology
Monophenol Monooxygenase
Organic Chemistry
General Medicine
Microphthalmia-associated transcription factor
Enzyme assay
Enzyme
chemistry
Cell culture
Pyrones
Cancer research
biology.protein
Kojic acid
Agaricales
Biotechnology
Transcription Factors
Subjects
Details
- ISSN :
- 13476947
- Volume :
- 74
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Bioscience, biotechnology, and biochemistry
- Accession number :
- edsair.doi.dedup.....fd6df7ba5a88ed9e1e5efb83f7603701