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Molecular markers for discriminating Streptococcus pyogenes and S. dysgalactiae subspecies equisimilis
- Source :
- European journal of clinical microbiologyinfectious diseases : official publication of the European Society of Clinical Microbiology. 29(5)
- Publication Year :
- 2009
-
Abstract
- Given the increasing aetiological importance of Streptococcus dysgalactiae subspecies equisimilis in diseases which are primarily attributed to S. pyogenes, molecular markers are essential to distinguish these species and delineate their epidemiology more precisely. Many clinical microbiology laboratories rely on agglutination reactivity and biochemical tests to distinguish them. These methods have limitations which are particularly exacerbated when isolates with mixed properties are encountered. In order to provide additional distinguishing parameters that could be used to unequivocally discriminate these two common pathogens, we assess here three molecular targets: the speB gene, intergenic region upstream of the scpG gene (IRSG) and virPCR. Of these, the former two respectively gave positive and negative results for S. pyogenes, and negative and positive results for S. dysgalactiae subsp. equisimilis. Thus, a concerted use of these nucleic acid-based methods is particularly helpful in epidemiological surveillance to accurately assess the relative contribution of these species to streptococcal infections and diseases.
- Subjects :
- Microbiology (medical)
Genetic Markers
medicine.medical_specialty
Streptococcus pyogenes
Agglutination
Subspecies
medicine.disease_cause
Polymerase Chain Reaction
Microbiology
Diagnosis, Differential
Intergenic region
Medical microbiology
Bacterial Proteins
Species Specificity
Streptococcal Infections
medicine
Humans
Gene
Microbiology & Cell Biology
biology
Streptococcus
General Medicine
Streptococcaceae
biology.organism_classification
Clinical microbiology
Cysteine Endopeptidases
Infectious Diseases
Subjects
Details
- ISSN :
- 14354373
- Volume :
- 29
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- European journal of clinical microbiologyinfectious diseases : official publication of the European Society of Clinical Microbiology
- Accession number :
- edsair.doi.dedup.....fd4e985f99a02fba0d3e7850c84106b2