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Expression system for production of bioactive compounds, recombinant human adiponectin, in the silk glands of transgenic silkworms
- Source :
- Archives of Pharmacal Research. 37:645-651
- Publication Year :
- 2013
- Publisher :
- Springer Science and Business Media LLC, 2013.
-
Abstract
- Adiponectin is an adipocyte hormone involved in glucose and lipid metabolism. The aim of this study was to develop a human adiponectin expression system in transgenic silkworm using a human adiponectin expression vector. The silk gland of the silkworm is a highly specialized organ that has the wonderful ability to synthesize and secrete silk protein. To express human adiponectin in the silk gland of transgenic silkworm, targeting vectors pB-A3-adiponectin-IRES-RFP and pB-Ser1-adiponectin-IRES-RFP were constructed and then introduced into the silkworm pupa. The transgenic silkworms were verified by PCR and then generated. The level of adiponectin in the transgenic silkworm was 6-10 ng/50 mg of freeze-dried powder, and western blotting using an antibody against human adiponectin demonstrated a specific band with a molecular weight of 30 kDa in the silkworm. These results showed that human adiponectin introduced into the silkworm genome was expressed successfully on a large-scale.
- Subjects :
- medicine.medical_specialty
Transgene
Blotting, Western
Genetic Vectors
Polymerase Chain Reaction
law.invention
Animals, Genetically Modified
chemistry.chemical_compound
Bombyx mori
law
Adipocyte
Internal medicine
Drug Discovery
medicine
Animals
Humans
Expression vector
biology
Adiponectin
fungi
Organic Chemistry
nutritional and metabolic diseases
Bombyx
biology.organism_classification
Recombinant Proteins
Genetically modified organism
Molecular Weight
Blot
Endocrinology
Gene Expression Regulation
chemistry
Biochemistry
Recombinant DNA
Molecular Medicine
Electrophoresis, Polyacrylamide Gel
hormones, hormone substitutes, and hormone antagonists
Subjects
Details
- ISSN :
- 19763786 and 02536269
- Volume :
- 37
- Database :
- OpenAIRE
- Journal :
- Archives of Pharmacal Research
- Accession number :
- edsair.doi.dedup.....fd2d5b1f7b2852a928ce3075dd8b19c9