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Biodegradation of neonicotinoid insecticide, imidacloprid by restriction enzyme mediated integration (REMI) generated Trichoderma mutants
- Source :
- Chemosphere. 112:526-530
- Publication Year :
- 2014
- Publisher :
- Elsevier BV, 2014.
-
Abstract
- REMI (restriction enzyme-mediated integration) technique was employed to construct Trichoderma atroviride strain T23 mutants with degrading capability of neonicotinoid insecticide, imidacloprid. The plasmid pBluescript II KS-hph used for integration in REMI mutants was confirmed by PCR and Southern hybridization. Among 153 transformants, 57% of them have showed higher neonicotinoid insecticide, imidacloprid, degradation ability than the wild strain T23 (p
- Subjects :
- Insecticides
Environmental Engineering
Health, Toxicology and Mutagenesis
Mutant
Microbiology
Neonicotinoids
chemistry.chemical_compound
Plasmid
Imidacloprid
Restriction enzyme mediated integration
Botany
Environmental Chemistry
Remi
Trichoderma
pBluescript
biology
Imidazoles
Public Health, Environmental and Occupational Health
Neonicotinoid
DNA Restriction Enzymes
General Medicine
General Chemistry
Nitro Compounds
biology.organism_classification
Pollution
Biodegradation, Environmental
chemistry
Environmental Pollutants
Genetic Engineering
Plasmids
Subjects
Details
- ISSN :
- 00456535
- Volume :
- 112
- Database :
- OpenAIRE
- Journal :
- Chemosphere
- Accession number :
- edsair.doi.dedup.....fd223f8679ace08917e5bc9a2f275e88
- Full Text :
- https://doi.org/10.1016/j.chemosphere.2014.01.023