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Strategies for intra-amniotic administration of fetal therapy in a rabbit model of intrauterine growth restriction
- Source :
- Exp Biol Med (Maywood)
- Publication Year :
- 2021
- Publisher :
- SAGE Publications, 2021.
-
Abstract
- Intrauterine growth restriction affects up to 10% of all pregnancies, leading to fetal programming with detrimental consequences for lifelong health. However, no therapeutic strategies have so far been effective to ameliorate these consequences. Our previous study has demonstrated that a single dose of nutrients administered into the amniotic cavity, bypassing the often dysfunctional placenta via intra-amniotic administration, improved survival at birth but not birthweight in an intrauterine growth restriction rabbit model. The aim of this study was to further develop an effective strategy for intra-amniotic fetal therapy in an animal model. Intrauterine growth restriction was induced by selective ligation of uteroplacental vessels on one uterine horn of pregnant rabbits at gestational day 25, and fetuses were delivered by cesarean section on GD30. During the five days of intrauterine growth restriction development, three different methods of intra-amniotic administration were used: continuous intra-amniotic infusion by osmotic pump, multiple intra-amniotic injections, and single fetal intraperitoneal injection. Technical feasibility, capability to systematically reach the fetus, and survival and birthweight of the derived offspring were evaluated for each technique. Continuous intra-amniotic infusion by osmotic pump was not feasible owing to the high occurrence of catheter displacement and amnion rupture, while methods using two intra-amniotic injections and one fetal intraperitoneal injection were technically feasible but compromised fetal survival. Taking into account all the numerous factors affecting intra-amniotic fetal therapy in the intrauterine growth restriction rabbit model, we conclude that an optimal therapeutic strategy with low technical failure and positive fetal impact on both survival and birthweight still needs to be found.
- Subjects :
- 0301 basic medicine
Catheters
Physiology
Intrauterine growth restriction
General Biochemistry, Genetics and Molecular Biology
03 medical and health sciences
0302 clinical medicine
Animals
Birth Weight
Medicine
Medical nutrition therapy
Fetal programming
Fetal therapy
Infusion Pumps
Original Research
Fetal Therapies
Fetal Growth Retardation
030219 obstetrics & reproductive medicine
business.industry
Nutrients
Transamniotic
Amniotic Fluid
medicine.disease
030104 developmental biology
Intra-Amniotic
Rabbit model
Female
Nutrition Therapy
Rabbits
business
Injections, Intraperitoneal
Subjects
Details
- ISSN :
- 15353699 and 15353702
- Volume :
- 246
- Database :
- OpenAIRE
- Journal :
- Experimental Biology and Medicine
- Accession number :
- edsair.doi.dedup.....fd128f5f06cacd8c2967e6957a70cd8d