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Oncospheral penetration glands are the source of the EG95 vaccine antigen against cystic hydatid disease
- Source :
- Parasitology. 138:89-99
- Publication Year :
- 2010
- Publisher :
- Cambridge University Press (CUP), 2010.
-
Abstract
- SUMMARYImmunohistochemistry and immunogold labelling techniques were used to localize the EG95 vaccine antigen in Echinococcus granulosus oncospheres. In non-activated oncospheres, the cytoplasm of 2 pairs of bilateral cells exhibited specific positive labelling for the presence of EG95. No surface localization was seen in non-activated or recently activated oncospheres. Besides the staining of 2 pairs of bilateral cells, there was also a generalized distribution of specific staining for EG95 throughout the parenchyma of activated oncospheres. Immunogold labelling of non-activated oncosphere revealed specific reactivity for EG95 involving 2 pairs of bilateral cells and the ultrastructural characteristics of these cells were consistent with them being penetration gland cells. No other oncospheral structures stained specifically for the presence of EG95. The absence of surface location of EG95 in oncospheres suggests that the parasite may not be susceptible to vaccine-induced antibody and complement mediated attack until some post-oncospheral development has occurred. Further studies would be required to determine when the EG95 antigen associates with the parasite's surface, thus making them susceptible to immune attack.
- Subjects :
- Pathology
medicine.medical_specialty
Biology
Host-Parasite Interactions
Immune system
Antigen
Echinococcosis
Parenchyma
medicine
Animals
Echinococcus granulosus
Vaccines
Sheep
Australia
Oncosphere
Helminth Proteins
Immunogold labelling
biology.organism_classification
Immunohistochemistry
Staining
Infectious Diseases
Antigens, Helminth
biology.protein
Animal Science and Zoology
Parasitology
Antibody
Subjects
Details
- ISSN :
- 14698161 and 00311820
- Volume :
- 138
- Database :
- OpenAIRE
- Journal :
- Parasitology
- Accession number :
- edsair.doi.dedup.....fd11a6016bcc1a0a7e187bcb89364cdc