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DNA barcoding in the rust genus Chrysomyxa and its implications for the phylogeny of the genus
- Source :
- Mycologia, Mycologia, Mycological Society of America, 2011, 103 (6), pp.1250-1266. ⟨10.3852/10-426⟩
- Publication Year :
- 2011
- Publisher :
- HAL CCSD, 2011.
-
Abstract
- International audience; Chrysomyxa rusts are fungal pathogens widely present in the boreal forest. Taxonomic delimitation and precise species identification are difficult within this genus because several species display similar morphological features. We applied a DNA barcode system based on the ribosomal internal transcribed spacer region (ITS), large subunit (28S) ribosomal RNA gene, mitochondrial cytochrome oxidase 1 (CO1) and mitochondrial NADH dehydrogenase subunit 6 (NAD6) in 86 strains from 16 different Chrysomyxa species, including members of the Chrysomyxa ledi species complex. The nuclear ITS and 28S loci revealed higher resolving power than the mitochondrial genes. Amplification of the full CO1 barcode region failed due to the presence of introns limiting the dataset obtained with this barcode. In most cases the ITS barcodes were in agreement with taxonomic species based on phenotypic characters. Nevertheless we observed genetically distinct (different DNA barcodes) lineages within Chrysomyxa pyrolae and Chrysomyxa rhododendri, providing some evidence for allopatric speciation within these morphologically defined species. This finding, together with the observed pattern of host specificities of the studied rust fungi, suggest that species diversification within the C. ledi species complex might be governed by a set of factors such as specialisation to certain Ericaceae species as telial hosts and to a lesser extent specialization to different spruce species as aecial hosts. Moreover allopatric speciation by geographic disruption of species also seems to take place. When our data were integrated into a broader phylogenetic framework the Chrysomyxa genus unexpectedly was not resolved as a monophyletic group. Indeed the spruce cone rusts C. pyrolae and C. monesis coalesced with the pine needle rusts belonging to the genus Coleosporium, whereas the microcyclic species Chrysomyxa weirii was embedded within a clade comprising the genus Melampsora.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Species complex
Physiology
Chrysomyxa pyrolae
phylogeny
010603 evolutionary biology
01 natural sciences
DNA barcoding
03 medical and health sciences
Genus
Botany
DNA, Ribosomal Spacer
Genetics
medicine
DNA barcode
mitochondrial heteroplasmon
Internal transcribed spacer
fungal pathogen
DNA, Fungal
species complex
Molecular Biology
Ecology, Evolution, Behavior and Systematics
[SDV.MP.MYC]Life Sciences [q-bio]/Microbiology and Parasitology/Mycology
Electronic Data Processing
Coleosporium
biology
Basidiomycota
cone rusts
Cell Biology
General Medicine
030108 mycology & parasitology
15. Life on land
biology.organism_classification
Chrysomyxa
chrysomyxa needle rusts
medicine.drug_formulation_ingredient
Chrysomyxa ledi
mycology
Subjects
Details
- Language :
- English
- ISSN :
- 00275514
- Database :
- OpenAIRE
- Journal :
- Mycologia, Mycologia, Mycological Society of America, 2011, 103 (6), pp.1250-1266. ⟨10.3852/10-426⟩
- Accession number :
- edsair.doi.dedup.....fd038f7784ac9b9ee281887772d04607